A colorimetric hydrogel biosensor for rapid detection of nitrite ions

被引：100
作者：
Nam, Jihye [1 ]
Jung, In-Bok [2 ]
Kim, Boyoon [1 ]
Lee, Sang-Myung [3 ]
Kim, Seong-Eun [2 ]
Lee, Kook-Nyung [2 ]
Shin, Dong-Sik [1 ]
机构：
[1] Sookmyung Womens Univ, Dept Chem & Biol Engn, Seoul 04310, South Korea
[2] KETI, Gyeonggi 13509, South Korea
[3] Kangwon Natl Univ, Dept Chem Engn, Gangwon Do 24341, South Korea
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2018年 / 270卷
基金：
新加坡国家研究基金会;
关键词：
Nitrite ions; Rapid detection; Griess assay; Glass fiber; Hydrogel; STRIP BIOSENSOR; ACETYLCHOLINESTERASE BIOSENSOR; SENSITIVE DETECTION; GRIESS REACTION; OXIDE SYNTHASE; NITRATE; FABRICATION; RELEASE; PROBE;
D O I：
10.1016/j.snb.2018.04.171
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Nitrite ions are important markers of inflammation, food safety, and water quality; therefore, various methods are under development to detect nitrite ions. This paper describes a novel method for detecting nitrite ions using polyethylene glycol (PEG) hydrogel superimposed glass fiber membrane strips. A colorimetric Griess assay was performed on the hydrogel modified with N-(1-naphthyl)ethylenediamine by dropping a solution of nitrite and sulfanilamide. This colorimetric hydrogel sensor exhibited a detection limit as low as 10 mu M for nitrite ions and the working concentration of nitrite ions was between 10 mu M and 5 mM. The colorimetric reaction was completed within a few seconds; the performance test results indicated that the sensor was specific to nitrite ions compared to other anions, and it was suitable to detect nitrite ions in clinical samples. In the future, this sensor platform can be used to detect nitrite ions in various complex solutions including biological and environmental samples. (C) 2018 Elsevier B.V. All rights reserved.
引用
收藏
页码：112 / 118
页数：7
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