Stilbenoids from Rheum undulatum Protect Hepatocytes Against Oxidative Stress Through AMPK Activation

被引：16
作者：
Dong, Guang-zhi [1 ,2 ]
Lee, Yu-ih [1 ,2 ]
Jeong, Ji Hye [1 ,2 ]
Zhao, Hui-Yuan [1 ,2 ]
Jeon, Raok [1 ,2 ]
Lee, Hwa Jin [3 ]
Ryu, Jae-Ha [1 ,2 ]
机构：
[1] Sookmyung Womens Univ, Res Ctr Cell Fate Control, Seoul 140742, South Korea
[2] Sookmyung Womens Univ, Coll Pharm, Seoul 140742, South Korea
[3] Semyung Univ, Dept Nat Med Resources, Jecheon 390711, Chungbuk, South Korea
来源：
PHYTOTHERAPY RESEARCH | 2015年 / 29卷 / 10期
基金：
新加坡国家研究基金会;
关键词：
Rheum undulatum; stilbenoid; hepatocyte protection; antioxidant; AMPK; ARACHIDONIC-ACID; FATTY LIVER; KINASE; PTEROSTILBENE; INHIBITION; APOPTOSIS; SIRTUINS; TARGET; MICE;
D O I：
10.1002/ptr.5442
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Oxidative stress promotes several diseases, including liver disease. We have isolated several stilbenoids from Rheum undulatum to investigate their hepatoprotective activities and mechanism. Stilbenoids from R. undulatum protects hepatocytes against arachidonic acid + iron (AA + Fe) induced oxidative stress. Pterostilbene (compound 5) shows stronger activity than the others. Trimethoxystilbenoid (compound 6) shows best activity on protection of HepG2 cells from AA + Fe-induced oxidative stress, and trans-stilbenoid (compound 7) shows weak activity. These stilbenoids suppress ROS generation in AA + Fe-treated HepG2 cells and also suppress AA + Fe-induced MMP disruption. Their protective effects on AA + Fe-induced MMP disruption were abrogated by treatment of AMP-activated protein kinase (AMPK) inhibitor, compound C or transfection of dominant negative form of AMPK. Taken together, stilbenoids from R. undulatum protect hepatocytes against AA + Fe-induced oxidative stress through AMPK activation. And the methoxy groups in the aryl groups are important for their cytoprotective activity. Copyright (c) 2015 John Wiley & Sons, Ltd.
引用
收藏
页码：1605 / 1609
页数：5
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