A gustatory receptor tuned to the steroid plant hormone brassinolide in Plutella xylostella (Lepidoptera: Plutellidae)

被引：40

作者：

Yang, Ke ^{[1
,2
]}

Gong, Xin-Lin ^{[1
,2
]}

Li, Guo-Cheng ^{[1
,2
]}

Huang, Ling-Qiao ^{[1
]}

Ning, Chao ^{[1
,2
]}

Wang, Chen-Zhu ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing, Peoples R China

[2] Univ Chinese Acad Sci, CAS Ctr Excellence Biot Interact, Beijing, Peoples R China

来源：

ELIFE | 2020年 / 9卷

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

DIAMONDBACK MOTH; BITTER TASTE; MOLECULAR EVOLUTION; CAFFEINE RESPONSE; CHEMORECEPTION; GENES; OVIPOSITION; EXPRESSION; GROWTH; LARVA;

D O I：

10.7554/eLife.64114

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Feeding and oviposition deterrents help phytophagous insects to identify host plants. The taste organs of phytophagous insects contain bitter gustatory receptors (GRs). To explore their function, the GRs in Plutella xylostella were analyzed. Through RNA sequencing and qPCR, we detected abundant PxylGr34 transcripts in the larval head and adult antennae. Functional analyses using the Xenopus oocyte expression system and 24 diverse phytochemicals showed that PxylGr34 is tuned to the canonical plant hormones brassinolide (BL) and 24-epibrassinolide (EBL). Electrophysiological analyses revealed that the medial sensilla styloconica of 4th instar larvae are responsive to BL and EBL. Dual-choice bioassays demonstrated that BL inhibits larval feeding and female oviposition. Knock-down of PxylGr34 by RNAi attenuates the taste responses to BL, and abolishes BL-induced feeding inhibition. These results increase our understanding of how herbivorous insects detect compounds that deter feeding and oviposition, and may be useful for designing plant hormone-based pest management strategies.

引用

页数：23

共 74 条

[31] Multiple gustatory receptors required for the caffeine response in Drosophila [J].