In vitro effects of 2-methoxyestradiol on cell numbers, morphology, cell cycle progression, and apoptosis induction in oesophageal carcinoma cells

被引:23
作者
Thaver, Veneesha [1 ,2 ]
Lottering, Mona-Liza [1 ]
van Papendorp, Dirk [1 ]
Joubert, Annie [1 ]
机构
[1] Univ Pretoria, Dept Physiol, ZA-0001 Pretoria, South Africa
[2] Univ Limpopo, Dept Physiol, Pretoria, South Africa
关键词
oesophageal carcinoma; 2-methoxyestradiol; metaphase blocks; apoptosis; ENDOGENOUS ESTROGEN METABOLITE; CANCER-CELLS; PANCREATIC-CANCER; EXPRESSION LEVELS; TUMOR-GROWTH; DEATH; MCF-7; ANGIOGENESIS; PATHWAY;
D O I
10.1002/cbf.1557
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The influence of 2-methoxyestradiol (2-ME) was investigated on cell numbers, morphology, cell cycle progression, and apoptosis induction in an oesophageal carcinoma cell line (WHCO3). Dose-dependent studies (1 X 10(-9) M-1 X 10(-6) M) revealed that 2-ME significantly reduced cell numbers to 60% in WHCO3 after 72 h of exposure at a concentration of 1 X 10(-6)M compared to vehicle-treated cells. Morphological studies entailing light-, fluorescent-, as well as transmission electron microscopy (TEM) confirmed 2-ME's antimitotic effects. These results indicated hallmarks of apoptosis including cell shrinkage, hypercondensation of chromatin, cell membrane blebbing, and apoptotic bodies in treated cells. Flow cytometric analyses demonstrated an increase in the G(2)/M-phase after 2-ME exposure; thus preventing cells from proceeding through the cell cycle. beta-tubulin immunofluorescence revealed that 2-ME caused spindle disruption. In addition, increased expression of death receptor 5 protein was observed further supporting the proposed mechanism of apoptosis induction via the extrinsic pathway in 2-ME-exposed oesophageal carcinoma cells. Copyright (C) 2009 John Wiley & Sons, Led.
引用
收藏
页码:205 / 210
页数:6
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[41]   Induction of apoptosis and G2/M arrest by 2-Methoxyestradiol in human cervical cancer HeLaS3 cells [J].
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[46]   p38 Mitogen-activated protein kinases is required for counteraction of 2-methoxyestradiol to estradiol-stimulated cell proliferation and induction of apoptosis in ovarian carcinoma cells via phosphorylation Bcl-2 [J].
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Hou, Y ;
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Wang, JW ;
Ni, X ;
Zheng, SS .
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Wen, Xian-Jie ;
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Morishita, Y ;
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