Cloning and characterization of Xenopus laevis xSox7 cDNA

被引：25
作者：
Shiozawa, M
Hiraoka, Y
Komatsu, N
Ogawa, M
Sakai, Y
Aiso, S
机构：
[1] KEIO UNIV, SCH MED, DEPT ANAT, SHINJUKU KU, TOKYO 160, JAPAN
[2] COSMO RES INST, BIOTECHNOL LAB, SATTE, SAITAMA 34001, JAPAN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1996年 / 1309卷 / 1-2期
关键词：
cDNA; nucleotide sequence; SOX; SRY; (Xenopus laevis);
D O I：
10.1016/S0167-4781(96)00145-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shift assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.
引用
收藏
页码：73 / 76
页数：4
相关论文
共 25 条
[21]   SOX3 IS AN X-LINKED GENE-RELATED TO SRY [J].
STEVANOVIC, M ;
LOVELLBADGE, R ;
COLLIGNON, J ;
GOODFELLOW, PN .
HUMAN MOLECULAR GENETICS, 1993, 2 (12) :2013-2018
[22]   EMBRYONIC EXPRESSION OF THE CHICKEN SOX2, SOX3 AND SOX11 GENES SUGGESTS AN INTERACTIVE ROLE IN NEURONAL DEVELOPMENT [J].
UWANOGHO, D ;
REX, M ;
CARTWRIGHT, EJ ;
PEARL, G ;
HEALY, C ;
SCOTTING, PJ ;
SHARPE, PT .
MECHANISMS OF DEVELOPMENT, 1995, 49 (1-2) :23-36
[23]   SOX-4, AN SRY-LIKE HMG BOX PROTEIN, IS A TRANSCRIPTIONAL ACTIVATOR IN LYMPHOCYTES [J].
VANDEWETERING, M ;
OOSTERWEGEL, M ;
VAN NORREN, K ;
CLEVERS, H .
EMBO JOURNAL, 1993, 12 (10) :3847-3854
[24]   AUTOSOMAL SEX REVERSAL AND CAMPOMELIC DYSPLASIA ARE CAUSED BY MUTATIONS IN AND AROUND THE SRY-RELATED GENE SOX9 [J].
WAGNER, T ;
WIRTH, J ;
MEYER, J ;
ZABEL, B ;
HELD, M ;
ZIMMER, J ;
PASANTES, J ;
BRICARELLI, FD ;
KEUTEL, J ;
HUSTERT, E ;
WOLF, U ;
TOMMERUP, N ;
SCHEMPP, W ;
SCHERER, G .
CELL, 1994, 79 (06) :1111-1120
[25]   THE HIGH-MOBILITY GROUP TRANSCRIPTION FACTOR, SOX4, TRANSACTIVATES THE HUMAN CD2 ENHANCER [J].
WOTTON, D ;
LAKE, RA ;
FARR, CJ ;
OWEN, MJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (13) :7515-7522
123