Cloning and characterization of Xenopus laevis xSox7 cDNA

被引：25
作者：
Shiozawa, M
Hiraoka, Y
Komatsu, N
Ogawa, M
Sakai, Y
Aiso, S
机构：
[1] KEIO UNIV, SCH MED, DEPT ANAT, SHINJUKU KU, TOKYO 160, JAPAN
[2] COSMO RES INST, BIOTECHNOL LAB, SATTE, SAITAMA 34001, JAPAN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1996年 / 1309卷 / 1-2期
关键词：
cDNA; nucleotide sequence; SOX; SRY; (Xenopus laevis);
D O I：
10.1016/S0167-4781(96)00145-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shift assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.
引用
收藏
页码：73 / 76
页数：4
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