Effects of LncRNA Lnc-LIF-AS on cell proliferation, migration and invasion in a human cervical cancer cell line

被引：6

作者：

Song, Weiguo ^{[1
]}

Wang, Juan ^{[1
]}

Liu, Hanyuan ^{[1
]}

Zhu, Chenchen ^{[1
]}

Xu, Fei ^{[1
]}

Qian, Lili ^{[2
]}

Shen, Zhen ^{[2
]}

Zhu, Jing ^{[2
]}

Yin, Shuai ^{[3
]}

Qin, Jiwei ^{[3
]}

Chen, Liang ^{[4
]}

Wu, Dabao ^{[2
]}

Nashan, Bjorn ^{[3
]}

Shan, Ge ^{[4
]}

Xiao, Weihua ^{[4
]}

Zhou, Ying ^{[1
]}

机构：

[1] Anhui Med Univ, Anhui Prov Hosp, Dept Obstet & Gynecol, Hefei 230001, Anhui, Peoples R China

[2] Univ Sci & Technol China, Anhui Prov Hosp, Affiliated Hosp 1, Dept Obstet & Gynecol, Hefei 230001, Anhui, Peoples R China

[3] Univ Sci & Technol China, Anhui Prov Hosp, Affiliated Hosp 1, Organ Transplantat Ctr, Hefei 230001, Anhui, Peoples R China

[4] Univ Sci & Technol China, CAS Key Lab Innate Immun & Chron Dis, Hefei Natl Lab Phys Sci Microscale, Sch Life Sci, Hefei 230027, Anhui, Peoples R China

来源：

CYTOKINE | 2019年 / 120卷

基金：

中国国家自然科学基金;

关键词：

LncRNA; Cervical cancer; Proliferation; Migration; Invasion; LONG NONCODING RNAS; STAT3; GENE; INFLAMMATION; METASTASIS; ACTIVATION; INSIGHTS; IL-6;

D O I：

10.1016/j.cyto.2019.05.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study explored the effect of LncRNA Lnc-LIF-AS on cell proliferation, migration and invasion in the human cervical cancer (HCC) cell line SiHa. SiHa cells had the lowest expression of Lnc-LIF-AS in the 4 human cervical cancer cell lines (SiHa, ME-180, C-33A and HeLa) and were transfected and divided into the SiHa/con (transfected with pMIGRI) cell group, SiHa/Lnc-LIF-AS (transfected with pMIGRI-Lnc-LIF-AS) cell group, and SiHa/Lnc-LIF-AS-DN (transfected with pMIGRI-Lnc-LIF-AS-DN, in which the sequences overlapping with LIF mRNA was deleted) cell group. Overexpression of Lnc-LIF-AS could promote the proliferation, colony formation, invasion and migration in SiHa and ME-180 cells. And the low expression of Lnc-LIF-AS suppress the proliferation, colony formation invasion and migration in HeLa cells when the Lnc-LIF-AS expression has been suppressed. In the SiHa/Lnc-LIF-AS cells group, the cell cycle was mainly halted in the S phase and overexpression of Lnc-LIF-AS had no effect on the apoptosis of SiHa cells. Overexpression of Lnc-LIF-AS could promote the secretion of LIF in SiHa cells, and the supernatant from SiHa/Lnc-LIF-AS cells could promote cell proliferation in the SiHa/con cells. The STAT3 inhibitor could inhibit cell proliferation in the SiHa/Lnc-LIF-AS cells. The expression level of Lnc-LIF-AS in cervical cancer tissues was higher than that in normal tissues and the expression level of Lnc-LIF-AS was positively correlated with the level of LIF. In the SiHa/con and SiHa/Lnc-LIF-AS-DN cell groups, there were no significant differences in cell proliferation, cell migration and cell invasion. The overexpression of Lnc-LIF-AS can promote cell proliferation, migration and invasion in cervical cancer cells, and the core function domain of this lncRNA was located in the overlapping a 3'-UTR base sequence of LIF mRNA.

引用

页码：165 / 175

页数：11

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