Bead array for Listeria monocytogenes detection using specific monoclonal antibodies

被引:12
作者
Karoonuthaisiri, Nitsara [1 ,2 ]
Charlermroj, Ratthaphol [1 ]
Teerapornpuntakit, Jarinthorn [3 ,4 ]
Kumpoosiri, Mallika [1 ]
Himananto, Orawan [1 ]
Grant, Irene R. [2 ]
Gajanandana, Oraprapai [1 ]
Elliott, Christopher T. [2 ]
机构
[1] Natl Sci & Technol Dev Agcy, Natl Ctr Genet Engn & Biotechnol BIOTEC, Pathum Thani, Thailand
[2] Queens Univ Belfast, Sch Biol Sci, Inst Global Food Secur, Belfast, Antrim, North Ireland
[3] Mahidol Univ, Fac Sci, Ctr Calcium & Bone Res COCAB, Bangkok 10400, Thailand
[4] Mahidol Univ, Fac Sci, Dept Physiol, Bangkok 10400, Thailand
关键词
Bead array; Listeria monocytogenes; Monoclonal antibody; Enzyme-linked immunosorbent assay; Multiplex detection; FOODBORNE PATHOGENS; ESCHERICHIA-COLI; IDENTIFICATION; BACTERIA; ASSAY; MICROARRAY; INFECTION; FLAGELLA; SYSTEM; VIRUS;
D O I
10.1016/j.foodcont.2014.07.049
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
To develop a detection method for human pathogenic Listeria monocytogenes, novel specific antibodies were obtained from hybridoma libraries generated by using formalist-killed and heat-killed L mono cytogenes as immunogens. Several monoclonal antibodies found to be specific to Listeria spp. or L monocytogenes were evaluated for their applicability as binders for bead array and sandwich ELISA for detection of L. monocytogenes in buffer and in 11 different food types. The bead array format consistently demonstrated lower detection limits and was less affected by interference from food matrices than the sandwich ELISA format. However, the obtained detection limits were not sufficient to satisfy the required standard for L. monocytogenes testing. Therefore, the international organization for standardization (ISO 11290-1:1996) methods for pre-enrichment and enrichment were employed to increase the bacteria numbers. When compared to the standard plating method, the bead array was able to detect the bacteria with the same accuracy even at the 1 CFU level after only 24 h of the enrichment period. In addition, Listeria-specific 3C3 and L. monocytogenes-specific 7G4 antibodies were successfully employed to construct a multiplex detection for Listeria, Salmonella and Campylobacter in a bead array format by combining with commercial Salmonella-specific and available Campylobacter-specific antibodies. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:462 / 471
页数:10
相关论文
共 43 条
[1]   ELISA DETECTION OF ODONTOGLOSSUM RINGSPOT VIRUS IN MATURE PLANTS AND PROTOCORMS OF CYMBIDIUM ORCHIDS - POTENTIAL SOLUTIONS TO PROBLEMS OF SAMPLE PREPARATION TIME AND LOW VIRUS CONCENTRATION [J].
ARUNASALAM, G ;
PEARSON, MN .
JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT, 1989, 126 (02) :160-166
[2]   Cold stress proteins induced in Listeria monocytogenes in response to temperature downshock and growth at low temperatures [J].
Bayles, DO ;
Annous, BA ;
Wilkinson, BJ .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (03) :1116-1119
[3]   Multiplex microsphere immuno-detection of potato virus Y, X and PLRV [J].
Bergervoet, Jan H. W. ;
Peters, Jeroen ;
van Beckhoven, Jose R. C. M. ;
van den Bovenkamp, Ge W. ;
Jacobson, James W. ;
van der Wolf, Jan M. .
JOURNAL OF VIROLOGICAL METHODS, 2008, 149 (01) :63-68
[4]   MONOCLONAL-ANTIBODY SPECIFIC FOR LISTERIA-MONOCYTOGENES ASSOCIATED WITH A 66-KILODALTON CELL-SURFACE ANTIGEN [J].
BHUNIA, AK ;
JOHNSON, MG .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (06) :1924-1929
[5]   METHODS AND MEDIA FOR THE ISOLATION AND CULTIVATION OF LISTERIA-MONOCYTOGENES FROM VARIOUS FOODS [J].
BRACKETT, RE ;
BEUCHAT, LR .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1989, 8 (03) :219-223
[6]   Multiplex Detection of Plant Pathogens Using a Microsphere Immunoassay Technology [J].
Charlermroj, Ratthaphol ;
Himananto, Orawan ;
Seepiban, Channarong ;
Kumpoosiri, Mallika ;
Warin, Nuchnard ;
Oplatowska, Michalina ;
Gajanandana, Oraprapai ;
Grant, Irene R. ;
Karoonuthaisiri, Nitsara ;
Elliott, Christopher T. .
PLOS ONE, 2013, 8 (04)
[7]   Comparison of techniques to screen and characterize bacteria-specific hybridomas for high-quality monoclonal antibodies selection [J].
Charlermroj, Ratthaphol ;
Oplatowska, Michalina ;
Kumpoosiri, Mallika ;
Himananto, Orawan ;
Gajanandana, Oraprapai ;
Elliott, Christopher T. ;
Karoonuthaisiri, Nitsara .
ANALYTICAL BIOCHEMISTRY, 2012, 421 (01) :26-36
[8]   A Chemiluminescent Antibody Array System for Detection of Foodborne Pathogens in Milk [J].
Charlermroj, Ratthaphol ;
Gajanandana, Oraprapai ;
Barnett, Charlie ;
Kirtikara, Kanyawim ;
Karoonuthaisiri, Nitsara .
ANALYTICAL LETTERS, 2011, 44 (06) :1085-1099
[9]   Simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Listeria monocytogenes with an array-based immunosorbent assay using universal protein G-liposomal nanovesicles [J].
Chen, CS ;
Durst, RA .
TALANTA, 2006, 69 (01) :232-238
[10]   PATHOGENICITY OF FOODBORNE, ENVIRONMENTAL AND CLINICAL ISOLATES OF LISTERIA-MONOCYTOGENES IN MICE [J].
CONNER, DE ;
SCOTT, VN ;
SUMNER, SS ;
BERNARD, DT .
JOURNAL OF FOOD SCIENCE, 1989, 54 (06) :1553-1556