A Single-Stranded Junction Modulates Nanosecond Motional Ordering of the Substrate Recognition Duplex of a Group I Ribozyme

被引：8
作者：
Phuong Nguyen [1 ]
Shi, Xuesong [2 ]
Sigurdsson, Snorri Th. [3 ]
Herschlag, Daniel [2 ]
Qin, Peter Z. [1 ]
机构：
[1] Univ So Calif, Dept Chem, Los Angeles, CA 90089 USA
[2] Stanford Univ, Dept Biochem, Stanford, CA 94305 USA
[3] Univ Iceland, Inst Sci, Dept Chem, IS-107 Reykjavik, Iceland
来源：
CHEMBIOCHEM | 2013年 / 14卷 / 14期
基金：
美国国家科学基金会;
关键词：
dynamics; EPR spectroscopy; motional ordering; RNA; spin labeling; ELECTRON-SPIN-RESONANCE; MOLECULAR-DYNAMICS SIMULATIONS; RNA DYNAMICS; SPECTRA; INTRON; COMPLEXITY; BINDING; DNA;
D O I：
10.1002/cbic.201300376
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Rigid spinning: Site-directed spin-labeling studies using a rigid nitroxide spin label (Ç) reveal that both length and sequence of a single-stranded junction (J1/2) modulate nanosecond motional ordering of the substrate-recognition duplex (P1) of the 120 kD group I ribozyme. The studies demonstrate an approach for experimental measurements of nanosecond dynamics in high-molecular-weight RNA complexes. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：1720 / 1723
页数：4
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