A llama single domain anti-idiotypic antibody mimicking HER2 as a vaccine: Immunogenicity and efficacy

被引：29

作者：

Alvarez-Rueda, Nidia ^{[1
]}

Ladjemi, Maha Zohra ^{[1
]}

Behar, Ghislaine ^{[2
]}

Corgnac, Stephanie ^{[1
]}

Pugniere, Martine ^{[3
]}

Roquet, Francoise ^{[3
]}

Bascoul-Mollevi, Caroline ^{[4
]}

Baty, Daniel ^{[5
]}

Pelegrin, Andre ^{[1
]}

Navarro-Teulon, Isabelle ^{[1
]}

机构：

[1] Univ Montpellier I, INSERM, U896, CRLC Val Aurelle Paul Lamarque,IRCM, F-34298 Montpellier 5, France

[2] CNRS, UMR 6204, Unite Biotechnol Biocatalyse & Bioregulat, F-44322 Nantes, France

[3] Ctr Etud Agents Pathogenes & Biotechnol Sante Pha, CNRS, UMR 5236, UM1,UM2, F-34093 Montpellier, France

[4] CRLC Val Aurelle Paul Lamarque, Unite Biostat, F-34298 Montpellier, France

[5] CNRS, UPR 9027, GDR 2352, Lab Ingn Syst Macromol, F-13402 Marseille, France

来源：

VACCINE | 2009年 / 27卷 / 35期

关键词：

Llama antibodies; HER2; Anti-idiotypic vaccine; EPITHELIAL OVARIAN-CANCER; METASTATIC BREAST-CANCER; ANTITUMOR PROPERTIES; MONOCLONAL-ANTIBODY; HER-2/NEU; ANTIGEN; TRASTUZUMAB; PEPTIDE; PROTEIN; HERCEPTIN((R));

D O I：

10.1016/j.vaccine.2009.05.067

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

HER2 over-expression in breast cancer correlates with reduced survival. Anti-idiotypic antibodies (Abs) that closely mimic HER2 could play a crucial role in the development of effective therapeutic vaccines. Here, we show that an anti-idiotypic single domain antibody (sdAb) 1HE isolated from a library generated from a Trastuzumab F(ab')(2)-immunized llama, closely mimics HER2. SdAb 1HE shows high affinity for Trastuzumab F(ab')(2), selectively inhibits HER2 binding to Trastuzumab F(ab')(2), and sera from sdAb 1HE-immunized BALB/c mice contain anti-HER2 antibodies that inhibit viability of HER2-positive cells. These results indicate that sdAb 1HE could be used as an anti-idiotype-based vaccine to boost immunity in patients bearing HER2-positive tumours. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：4826 / 4833

页数：8

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