Cloning, nucleotide sequencing, and expression of the β-galactosidase-encoding gene (lacA) from Aspergillus oryzae

被引：13

作者：

Ito, Y ^{[1
]}

Sasaki, T

Kitamoto, K

Kumagai, C

Takahashi, K

Gomi, K

Tamura, G

机构：

[1] Meiji Dairies Corp, Food Functional Res Inst, Lab Lact Acid Bacteria Res, Odawara 2500862, Japan

[2] Univ Tokyo, Dept Biotechnol, Bunkyo Ku, Tokyo 1138657, Japan

[3] Ozeki Corp, Gen Res Lab, Nishinomiya, Hyogo 6638227, Japan

[4] Takara Shuzo Co Ltd, Tech & Supplies Div, Kyoto 6008688, Japan

[5] Tohoku Univ, Grad Sch Agr Sci, Sendai, Miyagi 9818555, Japan

[6] Natl Inst Brewing Resources Co Ltd, Kita Ku, Tokyo 1140023, Japan

来源：

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY | 2002年 / 48卷 / 03期

关键词：

Aspergillus oryzae; beta-galactosidase; gene cloning; genomic gene; lacA;

D O I：

10.2323/jgam.48.135

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

lacA coding for beta-galactosidase (beta-gal) was cloned from the genomic DNA of Aspergillus oryzae RIB40. There were 9 exons in lacA and the coding region of 3,015 bp encoded a protein of 1,005 aa with a deduced molecular mass of 109,898. A. oryzae lacA was highly homologous to fungal beta-gals, with the highest aa identity of 70.7% to A. niger lacA, and also showed significant identity to acid beta-gals belonging to family 35 glycosyl hydrolases. Approximately 10 copies of lacA under control of A. oryzae glaA promoter were integrated into the chromosome of A. oryzae M-2-3. The recombinant strain expressed more than 700-fold of the beta-gal activity as compared to the wild type strain under induction by maltose.

引用

页码：135 / 142

页数：8

共 24 条

[1] ENZYMIC-SYNTHESIS OF DISACCHARIDES BY USE OF THE REVERSED HYDROLYSIS ACTIVITY OF BETA-D-GALACTOSIDASES [J].

AJISAKA, K ;

FUJIMOTO, H ;

NISHIDA, H .

CARBOHYDRATE RESEARCH, 1988, 180 (01) :35-42

[2] CHARACTERIZATION OF BETA-GALACTOSIDASE FROM A SPECIAL STRAIN OF ASPERGILLUS-ORYZAE [J].

AKASAKI, M ;

SUZUKI, M ;

FUNAKOSHI, I ;

YAMASHINA, I .

JOURNAL OF BIOCHEMISTRY, 1976, 80 (06) :1195-1200

[3] GALACTOSYLATION BY USE OF BETA-GALACTOSIDASE - CHEMOENZYMATIC SYNTHESES OF DISACCHARIDES AND TRISACCHARIDES [J].

BINDER, WH ;

KAHLIG, H ;

SCHMID, W .

TETRAHEDRON, 1994, 50 (35) :10407-10418

[4]

de Vries RP, 1999, APPL ENVIRON MICROB, V65, P2453

[5] A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].

DEVEREUX, J ;

HAEBERLI, P ;

SMITHIES, O .

NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395

[6]

GOMI K, 1987, AGR BIOL CHEM TOKYO, V51, P2549

[7] ANALYSIS OF A NOVEL GENE AND BETA-GALACTOSIDASE ISOZYME FROM A PSYCHROTROPHIC ARTHROBACTER ISOLATE [J].

GUTSHALL, KR ;

TRIMBUR, DE ;

KASMIR, JJ ;

BRENCHLEY, JE .

JOURNAL OF BACTERIOLOGY, 1995, 177 (08) :1981-1988

[8] FUNCTIONAL ELEMENTS OF THE PROMOTER REGION OF THE ASPERGILLUS-ORYZAE-GLAA GENE ENCODING GLUCOAMYLASE [J].

HATA, Y ;

KITAMOTO, K ;

GOMI, K ;

KUMAGAI, C ;

TAMURA, G .

CURRENT GENETICS, 1992, 22 (02) :85-91

[9] CONSERVED CATALYTIC MACHINERY AND THE PREDICTION OF A COMMON FOLD FOR SEVERAL FAMILIES OF GLYCOSYL HYDROLASES [J].

HENRISSAT, B ;

CALLEBAUT, I ;

FABREGA, S ;

LEHN, P ;

MORNON, JP ;

DAVIES, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (15) :7090-7094

[10] NEW FAMILIES IN THE CLASSIFICATION OF GLYCOSYL HYDROLASES BASED ON AMINO-ACID-SEQUENCE SIMILARITIES [J].

HENRISSAT, B ;

BAIROCH, A .

BIOCHEMICAL JOURNAL, 1993, 293 :781-788

← 1 2 3 →