Fragmentation behavior of metal-coded affinity tag (MeCAT)-labeled peptides

被引：21

作者：

Pieper, Stefan ^{[1
]}

Beck, Sebastian ^{[1
]}

Ahrends, Robert ^{[1
]}

Scheler, Christian ^{[2
]}

Linscheid, Michael W. ^{[1
]}

机构：

[1] Humboldt Univ, Dept Chem, D-12489 Berlin, Germany

[2] Proteome Factory AG, D-10117 Berlin, Germany

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2009年 / 23卷 / 13期

关键词：

ELECTRON-CAPTURE DISSOCIATION; MASS-SPECTROMETRY; PROTEINS; QUANTIFICATION; ACTIVATION; PROTEOMICS; MIXTURES; SILAC; IONS;

D O I：

10.1002/rcm.4118

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Quantitative proteomics has become an important method in modern life sciences. Besides protein identification, the aspect of quantification is of rapidly increasing relevance. MeCAT (metal-coded affinity tagging) is able to provide a tool that enables relative as well as absolute quantification. For structural elucidation, knowledge on the fragmentation behavior of MeCAT-modified peptides is highly beneficial. Therefore, the fragmentation behavior of MeCAT-labeled peptides under collision induced dissociation (CID), electron capture dissociation (ECD) and infrared multiphoton dissociation (IRMPD) conditions was studied. Application of CID and ECD allowed a straight-forward sequence elucidation of MeCAT-labeled peptides. During IRMPD all MeCAT-labeled peptides form characteristic fragments resulting from the fragmentational cleavage of the tagging group, thus, providing a screening method for the identification of labeled compounds. Furthermore, occurring side reactions during the labeling process were investigated. By-products were structurally characterized and reaction conditions were optimized in order to prevent the formation of these. Copyright (C) 2009 John Wiley & Sons, Ltd.

引用

页码：2045 / 2052

页数：8

共 19 条

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