Mycobacterium tuberculosis and whole-genome sequencing: how close are we to unleashing its full potential?

被引：90

作者：

Satta, G. ^{[1
,2
,3
]}

Lipman, M. ^{[4
,5
,6
]}

Smith, G. P. ^{[7
,8
]}

Arnold, C. ^{[1
,2
,9
]}

Kon, O. M. ^{[3
,10
]}

McHugh, T. D. ^{[1
,2
]}

机构：

[1] UCL, Dept Infect, UCL TB, London, England

[2] UCL, Dept Infect, UCL Ctr Clin Microbiol, London, England

[3] Imperial Coll Healthcare NHS Trust, London, England

[4] UCL, UCL TB, London, England

[5] UCL, UCL Resp, London, England

[6] Royal Free London NHS Fdn Trust, London, England

[7] Publ Hlth England, Natl Mycobacterium Reference Lab, London, England

[8] Heart England NHS Fdn Trust, Birmingham, W Midlands, England

[9] Publ Hlth England, Genom Serv & Dev Unit, London, England

[10] Imperial Coll, Natl Heart & Lung Inst, London, England

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2018年 / 24卷 / 06期

关键词：

Applications; Drug resistance; Mycobacterium tuberculosis; Whole genome sequencing; DRUG-RESISTANT-TUBERCULOSIS; GENES; IDENTIFICATION; MECHANISMS; PREDICTION; DIAGNOSIS; OUTBREAK; STRAINS; SAMPLES; IMPACT;

D O I：

10.1016/j.cmi.2017.10.030

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background: Nearly two decades after completion of the genome sequence of Mycobacterium tuberculosis (MTB), and with the advent of next generation sequencing technologies (NGS), whole-genome sequencing (WGS) has been applied to a wide range of clinical scenarios. Starting in 2017, England is the first country in the world to pioneer its use on a national scale for the diagnosis of tuberculosis, detection of drug resistance, and typing of MTB. Aims: This narrative review critically analyses the current applications of WGS for MTB and explains how close we are to realizing its full potential as a diagnostic, epidemiologic, and research tool. Sources: We searched for reports (both original articles and reviews) published in English up to 31 May 2017, with combinations of the following keywords: whole-genome sequencing, Mycobacterium, and tuberculosis. MEDLINE, Embase, and Scopus were used as search engines. We included articles that covered different aspects of whole-genome sequencing in relation to MTB. Content: This review focuses on three main themes: the role of WGS for the prediction of drug susceptibility, MTB outbreak investigation and genetic diversity, and research applications of NGS. (C) 2017 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

引用

页码：604 / 609

页数：6

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