Comparative analysis of Illumina Mouse Methylation BeadChip and reduced-representation bisulfite sequencing for routine DNA methylation analysis

被引：4

作者：

Fennell, Lochlan J. ^{[1
,2
,3
]}

Hartel, Gunter ^{[4
]}

McKeone, Diane M. ^{[1
]}

Bond, Catherine E. ^{[1
,3
]}

Kane, Alexandra ^{[1
,3
,5
]}

Leggett, Barbara A. ^{[1
,3
,7
]}

Patch, Ann-Marie ^{[6
]}

Whitehall, Vicki L. J. ^{[1
,3
,5
]}

机构：

[1] QIMR Berghofer Med Res Inst, Cell & Mol Biol Dept, Conjoint Gastroenterol Lab, Herston, Qld, Australia

[2] Monash Univ, Fac Med Nursing & Hlth Sci, Monash Biomed Discovery Inst, Anat & Dev Biol, 19 Innovat Walk, Clayton, Vic, Australia

[3] Univ Queensland, Fac Med, St Lucia, Qld, Australia

[4] QIMR Berghofer Med Res Inst, Stat Dept, Herston, Qld, Australia

[5] Pathol Queensland, Conjoint Internal Med Labs, Brisbane, Qld, Australia

[6] QIMR Berghofer Med Res Inst, Genet & Computat Biol Dept, Clin Genom Lab, Herston, Qld, Australia

[7] Royal Brisbane & Womens Hosp, Dept Gastroenterol & Hepatol, Brisbane, Qld, Australia

来源：

CELL REPORTS METHODS | 2022年 / 2卷 / 11期

关键词：

BRAF MUTATION; AGE;

D O I：

10.1016/j.crmeth.2022.100323

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Researching the murine epigenome in disease models has been hampered by the lack of appropriate and cost-effective DNA methylation arrays. Here we perform a comprehensive, comparative analysis between the Mouse Methylation BeadChip (MMB) and reduced-representation bisulfite sequencing (RRBS) in two murine models of colorectal carcinogenesis. We evaluate the coverage, variability, and ability to identify differential DNA methylation of RRBS and MMB. We show that MMB is an effective tool for profiling the murine methylome that performs comparably with RRBS, identifying similar differentially methylated pathways. Although choice of technology is experiment dependent and will be predicated on the underlying biology being probed, these analyses provide insights into the relative strengths and weaknesses of each approach.

引用

页数：13

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