Engineering of a bioluminescent antigen-binding protein

被引：14

作者：

Billiald, P

Mousli, M

Goyffon, M

Vaux, D

机构：

[1] UNIV OXFORD,SIR WILLIAM DUNN SCH PATHOL,OXFORD OX1 3RE,ENGLAND

[2] FAC PHARM TOURS,F-37200 TOURS,FRANCE

来源：

BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 10期

关键词：

D O I：

10.1023/A:1018463804651

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A gene fusion plasmid was constructed by inserting the structural gene of the firefly luciferase into a vector which allows expression of a functional recombinant scFv. The fusion protein which is expressed in the periplasm of bacteria, retains dual activity and functions in bioluminescent immunoassays. Such a genetic approach offers many advantages over chemical cross-linking of antibodies to colorimetric enzymes and may be adaptable to clinical immunoassays and high through-put drug screening assays.

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收藏

页码：1037 / 1041

页数：5

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