Effect of Oxidized Dextran on Cytokine Production and Activation of IRF3 Transcription Factor in Macrophages from Mice of Opposite Strains with Different Sensitivity to Tuberculosis Infection

被引:2
作者
Chechushkov, A. V. [1 ]
Kozhin, P. M. [1 ]
Zaitseva, N. S. [1 ]
Gainutdinov, P. I. [1 ]
Men'shchikova, E. B. [1 ]
Troitskii, A. V. [1 ]
Shkurupy, V. A. [1 ]
机构
[1] Res Inst Expt & Clin Med, Novosibirsk, Russia
关键词
60-kDa oxidized dextran; peritoneal macrophages; cytokines; C56Bl/6; CBA/J; SYSTEM;
D O I
10.1007/s10517-018-4070-5
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
We studied differences in the production of pro- and anti-inflammatory cytokines and IRF3 transcription factor by peritoneal macrophages from mice of opposite strains CBA/J and C57Bl/6 and the effect of 60-kDa oxidized dextran on these parameters. Macrophages from C57Bl/6 mice were mainly characterized by the production of proinflammatory cytokines TNF alpha, IL-12, and MCP-1 (markers of M1 polarization). By contrast, CBA/J mice exhibited a relatively high level of anti-inflammatory cytokine IL-10 and lower expression of proinflammatory cytokines (M2 phenotype). IRF3 content in peritoneal macrophages of CBA/J mice was higher than in C57Bl/6 mice. Oxidized dextran decreased the expression of IRF3 upon stimulation of cells from CBA/J mice with LPS, but increased this process in C57Bl/6 mice. Despite a diversity of oxidized dextran-induced changes in cytokine production, the data confirm our hypothesis that this agent can stimulate the alternative activation of macrophages.
引用
收藏
页码:738 / 742
页数:5
相关论文
共 15 条
[1]   Differences in the modulation of reactive species, lipid bodies, cyclooxygenase-2, 5-lipoxygenase and PPAR-γ in cerebral malaria-susceptible and resistant mice [J].
Borges, Tatiana K. S. ;
Alves, Erica A. R. ;
Vasconcelos, Henda A. R. ;
Carneiro, Fabiana P. ;
Nicola, Andre M. ;
Magalhaes, Kelly G. ;
Muniz-Junqueira, Maria Imaculada .
IMMUNOBIOLOGY, 2017, 222 (04) :604-619
[2]   Oxidized Dextran Enhances Alternative Activation of Macrophages in Mice of Opposite Lines [J].
Chechushkov, A. V. ;
Kozhin, P. M. ;
Zaitseva, N. S. ;
Lemza, A. E. ;
Men'shchikova, E. B. ;
Troitskii, A. V. ;
Shkurupy, V. A. .
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE, 2016, 160 (06) :783-786
[3]   Strain-dependent and distinctive T-cell responses to HIV antigens following immunisation of mice with differing chimpanzee adenovirus vaccine vectors [J].
Herath, S. ;
Le Heron, A. ;
Colloca, S. ;
Patterson, S. ;
Tatoud, R. ;
Weber, J. ;
Dickson, G. .
VACCINE, 2016, 34 (37) :4378-4385
[4]   Cytokine dysregulation induced by apoptotic cells is a shared characteristic of murine lupus [J].
Koh, JS ;
Wang, ZY ;
Levine, JS .
JOURNAL OF IMMUNOLOGY, 2000, 165 (08) :4190-4201
[5]   Mycobacterium Tuberculosis Activates the DNA-Dependent Cytosolic Surveillance Pathway within Macrophages [J].
Manzanillo, Paolo S. ;
Shiloh, Michael U. ;
Portnoy, Daniel A. ;
Cox, Jeffery S. .
CELL HOST & MICROBE, 2012, 11 (05) :469-480
[6]   Response of mononuclear phagocyte system to experimental tuberculosis in mice of opposite strains [J].
Nadeev, AP ;
Shkurupii, VA ;
Uvarova, TA ;
Pozdnyakova, SV .
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE, 2005, 140 (02) :253-256
[7]   Mycobacterium tuberculosis Triggers Host Type I IFN Signaling To Regulate IL-1β Production in Human Macrophages [J].
Novikov, Aleksey ;
Cardone, Marco ;
Thompson, Robert ;
Shenderov, Kevin ;
Kirschman, Kevin D. ;
Mayer-Barber, Katrin D. ;
Myers, Timothy G. ;
Rabin, Ronald L. ;
Trinchieri, Giorgio ;
Sher, Alan ;
Feng, Carl G. .
JOURNAL OF IMMUNOLOGY, 2011, 187 (05) :2540-2547
[8]   A comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection [J].
Probst, Christian M. ;
Silva, Rodrigo A. ;
Menezes, Juliana P. B. ;
Almeida, Tais F. ;
Gomes, Ivana N. ;
Dallabona, Andreia C. ;
Ozaki, Luiz S. ;
Buck, Gregory A. ;
Pavoni, Daniela P. ;
Krieger, Marco A. ;
Veras, Patricia S. T. .
BMC MICROBIOLOGY, 2012, 12
[9]   The Cell Wall Lipid PDIM Contributes to Phagosomal Escape and Host Cell Exit of Mycobacterium tuberculosis [J].
Quigley, Jeff ;
Hughitt, V. Keith ;
Velikovsky, Carlos A. ;
Mariuzza, Roy A. ;
El-Sayed, Najib M. ;
Briken, Volker .
MBIO, 2017, 8 (02)
[10]   Immunological Variation Between Inbred Laboratory Mouse Strains: Points to Consider in Phenotyping Genetically Immunomodified Mice [J].
Sellers, R. S. ;
Clifford, C. B. ;
Treuting, P. M. ;
Brayton, C. .
VETERINARY PATHOLOGY, 2012, 49 (01) :32-43