Identification of white clover (Trifolium repens L.) cultivars using molecular markers

被引：4
|
作者：
Dolanská, L [1 ]
Curn, V [1 ]
机构：
[1] Jihoceska Univ V Ceskych Budejovicich, Zemedelska Fak, Studentska 13, Ceske Budejovice 37005, Czech Republic
来源：
PLANT SOIL AND ENVIRONMENT | 2004年 / 50卷 / 03期
关键词：
Trifolium repens L; identification of cultivars; RAPD; microsatellites; rDNA; PCR-RFLP;
D O I：
10.17221/4013-PSE
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
The different molecular analysis for specification of white clover (Trifolium repens L.) populations was studied between 2002 and 2003. RAPD, SSR (microsatellites), rDNA and PCR-RFLP markers were used for this study. The high genetic variation was detected among the cultivars but also within the cultivars by RAPD markers. For this reason RAPD markers were not found as a suitable marker system for determination of white clover cultivars. The distribution of low genetic variation of rDNA and PCR-RFLP markers was not able to differentiate cultivars. SSR and rDNA markers did not show variability of patterns within one cultivar. The different sizes of PCR fragments were obtained after amplification with microsatellite primers. SSR markers are therefore suggested as the suitable markers for the identification of different T. repens cultivars.
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页码：95 / 100
页数：6
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