Identification of white clover (Trifolium repens L.) cultivars using molecular markers

被引：4

作者：

Dolanská, L ^{[1
]}

Curn, V ^{[1
]}

机构：

[1] Jihoceska Univ V Ceskych Budejovicich, Zemedelska Fak, Studentska 13, Ceske Budejovice 37005, Czech Republic

来源：

PLANT SOIL AND ENVIRONMENT | 2004年 / 50卷 / 03期

关键词：

Trifolium repens L; identification of cultivars; RAPD; microsatellites; rDNA; PCR-RFLP;

D O I：

10.17221/4013-PSE

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The different molecular analysis for specification of white clover (Trifolium repens L.) populations was studied between 2002 and 2003. RAPD, SSR (microsatellites), rDNA and PCR-RFLP markers were used for this study. The high genetic variation was detected among the cultivars but also within the cultivars by RAPD markers. For this reason RAPD markers were not found as a suitable marker system for determination of white clover cultivars. The distribution of low genetic variation of rDNA and PCR-RFLP markers was not able to differentiate cultivars. SSR and rDNA markers did not show variability of patterns within one cultivar. The different sizes of PCR fragments were obtained after amplification with microsatellite primers. SSR markers are therefore suggested as the suitable markers for the identification of different T. repens cultivars.

引用

页码：95 / 100

页数：6

共 22 条

[1] [Anonymous], 1990, PCR PROTOCOLS, DOI DOI 10.1016/B978-0-12-372180-8.50042-1
[2] The 5S rRNA gene in sea barley (Hordeum marinum Hudson sensu lato):: sequence variation among repeat units and relationship to the X haplome in barley (Hordeum)
Baum, BR
Johnson, DA
[J]. GENOME, 1998, 41 (05) : 652 - 661
[3] DNA AMPLIFICATION FINGERPRINTING USING VERY SHORT ARBITRARY OLIGONUCLEOTIDE PRIMERS
CAETANOANOLLES, G
BASSAM, BJ
GRESSHOFF, PM
[J]. BIO-TECHNOLOGY, 1991, 9 (06): : 553 - 557
[4] Consensus multiplex PCR-restriction fragment length polymorphism (RFLP) for rapid detection of plant mitochondrial DNA polymorphism
Chen, H
Sun, M
[J]. MOLECULAR ECOLOGY, 1998, 7 (11) : 1553 - 1556
[5] DOLANSKA L, 2002, SER CROP SCI, V19, P91
[6] GENETIC SEGREGATION OF RANDOM AMPLIFIED POLYMORPHIC DNA IN DIPLOID CULTIVATED ALFALFA
ECHT, CS
ERDAHL, LA
MCCOY, TJ
[J]. GENOME, 1992, 35 (01) : 84 - 87
[7] Genetic variation within and among white clover populations from managed permanent pastures of the northeastern USA
Gustine, DL
Huff, DR
[J]. CROP SCIENCE, 1999, 39 (02) : 524 - 530
[8] Microsatellites, from molecules to populations and back
Jarne, P
Lagoda, PJL
[J]. TRENDS IN ECOLOGY & EVOLUTION, 1996, 11 (10) : 424 - 429
[9] PORTRAYING OF PLANT GENOMES USING POLYMERASE CHAIN-REACTION AMPLIFICATION OF RIBOSOMAL 5S GENES
KOLCHINSKY, A
KOLESNIKOVA, M
ANANIEV, E
[J]. GENOME, 1991, 34 (06) : 1028 - 1031
[10] Development and characterisation of simple sequence repeat (SSR) markers for white clover (Trifolium repens L.)
Kölliker, R
Jones, ES
Drayton, MC
Dupal, MP
Forster, JW
[J]. THEORETICAL AND APPLIED GENETICS, 2001, 102 (2-3) : 416 - 424

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