Phytochemical analysis of the methanolic extract of Seriphidium oliverianum resulted in the isolation of eleven compounds namely: 5,8-dihydroxy-6,7,4'-trimethoxyflavone (1), 5-hydroxy-6,7,4'-trimethoxyflavone (2), 5,4'-dihydroxy-6,7-dimethoxyflavone (3), 5,7-dihydroxy-6,3',4'-trimethoxyflavone (4), 5,6-dihydroxy-7,3', 4'-trimethoxyflavone (5), 5,7,4'-tihydroxy-6,3'-dimethoxyflavone (6), 5,6,4'-trihydroxy-7,3'-dimethoxyflavone (7), 5,3',4'-trihydroxy-6,7-dimethoxyflavone (8), 5,7,3',4'-tetrahydroxy-3,6-dimethoxyflavone (9), 7,4'-dihydroxy-3,5,6-trimethoxyflavone (10) and 3,6-O-dimethylquercetagetin-7-O-beta-D-glucoside (11) for the first time from this source. Their structures were established through to spectroscopic analyses and in comparison with the literature. All the isolates were tested for their antioxidant and anti-urease activities.