Circadian Gene Profiling in Laser Capture Microdissected Mouse Club Cells

被引：0

作者：

Zhang, Zhenguang ^{[1
,2
]}

Loudon, Andrew ^{[1
]}

机构：

[1] Univ Manchester, Fac Biol Med & Hlth, Sch Med Sci, Manchester, Lancs, England

[2] Univ Cambridge, Dept Oncol, Hutchison MRC Res Bldg, Cambridge, England

来源：

BIO-PROTOCOL | 2020年 / 10卷 / 08期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Club cell; Lung; Circadian clock; RNA seq; Laser capture micro-dissection; LUNG;

D O I：

10.21769/BioProtoc.3590

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cell heterogeneity is high in tissues like lung. Research conducted on pure population of cells usually offers more insights than bulk tissues, such as circadian clock work. In this protocol, we provide a detailed work flow on how to do circadian clock study by RNA seq in laser capture micro-dissected mouse lung club cells. The method uses frozen tissues and is highly reproduciable.

引用

页数：8

共 8 条

[1] Laser capture microdissection and mRNA characterization of mouse airway epithelium: methodological considerations [J].

Betsuyaku, T ;

Senior, RM .

MICRON, 2004, 35 (04) :229-234

[2] A robust RNA integrity-preserving staining protocol for laser capture microdissection of endometrial cancer tissue [J].

Cummings, Michele ;

McGinley, Ciara V. ;

Wilkinson, Nafisa ;

Field, Sarah L. ;

Duffy, Sean R. ;

Orsi, Nicolas M. .

ANALYTICAL BIOCHEMISTRY, 2011, 416 (01) :123-125

[3] Circadian Timing in the Lung; A Specific Role for Bronchiolar Epithelial Cells [J].

Gibbs, J. E. ;

Beesley, S. ;

Plumb, J. ;

Singh, D. ;

Farrow, S. ;

Ray, D. W. ;

Loudon, A. S. I. .

ENDOCRINOLOGY, 2009, 150 (01) :268-276

[4] An epithelial circadian clock controls pulmonary inflammation and glucocorticoid action [J].

Gibbs, Julie ;

Ince, Louise ;

Matthews, Laura ;

Mei, Junjie ;

Bell, Thomas ;

Yang, Nan ;

Saer, Ben ;

Begley, Nicola ;

Poolman, Toryn ;

Pariollaud, Marie ;

Farrow, Stuart ;

DeMayo, Francesco ;

Hussell, Tracy ;

Worthen, G. Scott ;

Ray, David ;

Loudon, Andrew .

NATURE MEDICINE, 2014, 20 (08) :919-926

[5] Circadian rhythm reprogramming during lung inflammation [J].

Haspel, Jeffrey A. ;

Chettimada, Sukrutha ;

Shaik, Rahamthulla S. ;

Chu, Jen-Hwa ;

Raby, Benjamin A. ;

Cernadas, Manuela ;

Carey, Vincent ;

Process, Vanessa ;

Hunninghake, G. Matthew ;

Ifedigbo, Emeka ;

Lederer, James A. ;

Englert, Joshua ;

Pelton, Ashley ;

Coronata, Anna ;

Fredenburgh, Laura E. ;

Choi, Augustine M. K. .

NATURE COMMUNICATIONS, 2014, 5

[6] Considerations for RNA-seq Analysis of Circadian Rhythms [J].

Li, Jiajia ;

Grant, Gregory R. ;

Hogenesch, John B. ;

Hughes, Michael E. .

CIRCADIAN RHYTHMS AND BIOLOGICAL CLOCKS, PT A, 2015, 551 :349-367

[7] Light-dark oscillations in the lung transcriptome: implications for lung homeostasis, repair, metabolism, disease, and drug action [J].

Sukumaran, Siddharth ;

Jusko, William J. ;

DuBois, Debra C. ;

Almon, Richard R. .

JOURNAL OF APPLIED PHYSIOLOGY, 2011, 110 (06) :1732-1747

[8] A circadian gene expression atlas in mammals: Implications for biology and medicine [J].

Zhang, Ray ;

Lahens, Nicholas F. ;

Ballance, Heather I. ;

Hughes, Michael E. ;

Hogenesch, John B. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2014, 111 (45) :16219-16224

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