Structure of the DNA-Binding and RNA-Polymerase-Binding Region of Transcription Antitermination Factor λQ

被引：11

作者：

Vorobiev, Sergey M. ^{[1
]}

Gensler, Yocheved ^{[2
,3
]}

Vahedian-Movahed, Hanif ^{[3
,4
]}

Seetharaman, Jayaraman ^{[1
]}

Su, Min ^{[1
]}

Huang, Janet Y. ^{[5
,6
]}

Xiao, Rong ^{[5
,6
]}

Kornhaber, Gregory ^{[5
,6
]}

Montelione, Gaetano T. ^{[5
,6
]}

Tong, Liang ^{[1
]}

Ebright, Richard H. ^{[3
,4
]}

Nickels, Bryce E. ^{[2
,3
]}

机构：

[1] Columbia Univ, Northeast Struct Genom Consortium, Dept Biol Sci, New York, NY 10027 USA

[2] Rutgers State Univ, Dept Genet, Piscataway, NJ 08854 USA

[3] Rutgers State Univ, Waksman Inst, Piscataway, NJ 08854 USA

[4] Rutgers State Univ, Dept Chem, Piscataway, NJ 08854 USA

[5] Rutgers State Univ, Robert Wood Johnson Med Sch, Ctr Adv Biotechnol & Med, Piscataway, NJ 08854 USA

[6] Rutgers State Univ, Northeast Struct Genom Consortium, Piscataway, NJ 08854 USA

来源：

STRUCTURE | 2014年 / 22卷 / 03期

基金：

美国国家卫生研究院;

关键词：

FLAP DOMAIN; PROTEIN; SIGMA(70); SUBUNIT; HOLOENZYME; ELONGATION;

D O I：

10.1016/j.str.2013.12.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The bacteriophage lambda Q protein is a transcription anti-termination factor that controls expression of the phage late genes as a stable component of the transcription elongation complex. To join the elongation complex, lambda Q binds a specific DNA sequence element and interacts with RNA polymerase that is paused during early elongation. lambda Q binds to the paused early-elongation complex through interactions between lambda Q and two regions of RNA polymerase: region 4 of the sigma(70) subunit and the flap region of the beta subunit. We present the 2.1 angstrom resolution crystal structure of a portion of lambda Q containing determinants for interaction with DNA, interaction with region 4 of sigma(70), and interaction with the beta flap. The structure provides a framework for interpreting prior genetic and biochemical analysis and sets the stage for future structural studies to elucidate the mechanism by which lambda Q alters the functional properties of the transcription elongation complex.

引用

页码：488 / 495

页数：8

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