Stage-specific transcriptomic analysis of the model cestode Hymenolepis microstoma

被引:15
|
作者
Preza, Matias [1 ]
Calvelo, Javier [1 ,2 ]
Langleib, Mauricio [2 ]
Hoffmann, Federico [3 ]
Castillo, Estela [4 ]
Koziol, Uriel [1 ]
Iriarte, Andres [2 ]
机构
[1] Univ Republica, Fac Ciencias, Secc Biol Celular, Montevideo 11400, Uruguay
[2] Univ Republica, Inst Higiene, Fac Med, Dept Desarrollo Biotecnol,Lab Biol Computat, Montevideo 11600, Uruguay
[3] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Mississippi State, MS 39762 USA
[4] Univ Republica, Fac Ciencias, Secc Bioquim, Montevideo 11400, Uruguay
关键词
Tapeworm; RNA-seq; Flatworms functional genomics; Gene family evolution; Antigen B; LIGAND-BINDING PROTEIN; DIFFERENTIAL EXPRESSION; MESOCESTOIDES-CORTI; RAT TAPEWORM; ASCARIS-SUUM; COMPLEX-II; GENOME; METABOLISM; ADULT; ANNOTATION;
D O I
10.1016/j.ygeno.2021.01.005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Most parasitic flatworms go through different life stages with important physiological and morphological changes. In this work, we used a transcriptomic approach to analyze the main life-stages of the model tapeworm Hymenolepis microstoma (eggs, cysticercoids, and adults). Our results showed massive transcriptomic changes in this life cycle, including key gene families that contribute substantially to the expression load in each stage. In particular, different members of the cestode-specific hydrophobic ligand-binding protein (HLBP) family are among the most highly expressed genes in each life stage. We also found the transcriptomic signature of major metabolic changes during the transition from cysticercoids to adult worms. Thus, this work contributes to uncovering the gene expression changes that accompany the development of this important cestode model species, and to the best of our knowledge represents the first transcriptomic study with robust replicates spanning all of the main life stages of a tapeworm.
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页码:620 / 632
页数:13
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