Structure of a new 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose-containing O-specific polysaccharide from the lipopolysaccharide of Citrobacter gillenii PCM 1542

被引：2
作者：
Katzenellenbogen, E
Zatonsky, GV
Kocharova, NA
Bogulska, M
Korzeniowska-Kowal, A
Shashkov, AS
Gamian, A
Knirel, YA
机构：
[1] Polish Acad Sci, L Hirszfeld Inst Immunol & Expt Therapy, PL-53114 Wroclaw, Poland
[2] Russian Acad Sci, ND Zelinskii Organ Chem Inst, Moscow 119991, Russia
来源：
CARBOHYDRATE RESEARCH | 2002年 / 337卷 / 17期
基金：
俄罗斯基础研究基金会;
关键词：
Citrobacter gillenii; enterobacteria; O-antigen; lipopolysaccharide; bacterial polysaccharide structure; serological classification; H-1; C-13; NMR;
D O I：
10.1016/S0008-6215(02)00264-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The O-specific polysaccharide of Citrobacter gillenii PCM 1542 from serotype O-12a,12b is composed of one residue each of D-glucose, D-GlcNAc, 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose (D-GlcNAcyl) and two GalNAc residues. On the basis of sugar and methylation analyses of the intact and Smith degraded polysaccharides, along with ID and 2D H-1 and C-13 NMR spectroscopy, the following structure of the branched pentasaccharide repeating unit of the O-specific polysaccharide was established: [GRAPHICS] This structure differs significantly from that of the O-specific polysaccharide of C. gillenii PCM 1544 from the same serotype O-12a,12b, which has been established earlier (Kubler-Kielb, J. et al. Carbohydr. Res. 2001, 331, 331-336). Serological studies confirmed that the two O-antigens are not related and suggested that strains PCM 1542 and 1544 should be classified into different O-serogroups. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：1541 / 1546
页数：6
相关论文
共 37 条
1234