Isolation and characterization of microsatellite loci for the Asian longhorned beetle, Anoplophora glabripennis

被引：6

作者：

Carter, Maureen ^{[1
]}

Casa, Alexandra M. ^{[3
]}

Zeid, Mahmoud ^{[3
]}

Mitchell, Sharon E. ^{[3
]}

Kresovich, Stephen ^{[2
]}

机构：

[1] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA

[2] Cornell Univ, Dept Plant Breeding, Ithaca, NY 14853 USA

[3] Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA

来源：

MOLECULAR ECOLOGY RESOURCES | 2009年 / 9卷 / 03期

关键词：

Anoplophora glabripennis; Asian longhorned beetle; Coleoptera; SSR; UNITED-STATES; STRATEGIES; COLEOPTERA;

D O I：

10.1111/j.1755-0998.2008.02485.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the first isolation of SSR (simple sequence repeat) markers for Anoplophora glabripennis, a pest of forests in China and urban trees in the USA. Ten markers were developed using FIASCO (fast isolation by AFLP of sequences containing repeats) with an (AC)(17) probe, and five markers were identified in expressed sequence tags found on the NCBI database. Twenty-seven beetles from a population in Gansu Province, China, were assayed. The number of alleles ranged from 3 to 9; observed and expected heterozygosities ranged from 0.07 to 0.79 and from 0.30 to 0.78, respectively.

引用

页码：925 / 928

页数：4

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