A fluorescent probe for specific detection of cysteine in the lipid dense region of cells

被引:53
作者
Ali, Firoj [1 ]
Anila, H. A. [1 ]
Taye, Nandaraj [2 ]
Gonnade, Rajesh G. [3 ]
Chattopadhyay, Samit [2 ]
Das, Amitava [1 ]
机构
[1] CSIR, Natl Chem Lab, Div Organ Chem, Pune 411007, Maharashtra, India
[2] Natl Ctr Cell Sci, Chromatin & Dis Biol Lab, Pune 411007, Maharashtra, India
[3] CSIR, Natl Chem Lab, Ctr Mat Characterizat, Pune 411007, Maharashtra, India
关键词
DISULFIDE BOND FORMATION; GLUTATHIONE; CHEMODOSIMETER; HOMOCYSTEINE; REAGENTS; THIOLS; STATE; ASSAY; DYE;
D O I
10.1039/c5cc07450a
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A new cysteine (Cys) specific chemodosimetric reagent (ER-F) is used in imaging of endogenous Cys localized in the lipid dense region of the live Hct116 cells and the release of Cys within HepG2 cells from a drug following a biochemical transformation. A silica surface, modified with ER-F, could be used for quantitative estimation of Cys present in aqueous solution (pH 7.2) and in a human blood plasma (HBP).
引用
收藏
页码:16932 / 16935
页数:4
相关论文
共 43 条
[1]   A reagent for specific recognition of cysteine in aqueous buffer and in natural milk: imaging studies, enzymatic reaction and analysis of whey protein [J].
Anila, H. A. ;
Reddy, Upendar G. ;
Ali, Firoj ;
Taye, Nandaraj ;
Chattopadhyay, Samit ;
Das, Amitava .
CHEMICAL COMMUNICATIONS, 2015, 51 (85) :15592-15595
[2]   The in vivo sparing of methionine by cysteine in sulfur amino acid requirements in animal models and adult humans [J].
Ball, Ronald O. ;
Courtney-Martin, Glenda ;
Pencharz, Paul B. .
JOURNAL OF NUTRITION, 2006, 136 (06) :1682S-1693S
[3]   IDENTIFICATION OF A PROTEIN REQUIRED FOR DISULFIDE BOND FORMATION INVIVO [J].
BARDWELL, JCA ;
MCGOVERN, K ;
BECKWITH, J .
CELL, 1991, 67 (03) :581-589
[4]   Solvent and pH dependent fluorescent properties of a dimethylaminostyryl borondipyrromethene dye in solution [J].
Baruah, Mukulesh ;
Qin, Wenwu ;
Flors, Cristina ;
Hofkens, Johan ;
Valle, Renaud A. L. ;
Beljonne, David ;
Van der Auweraer, Mark ;
De Borggraeve, Wim M. ;
Boens, Noel .
JOURNAL OF PHYSICAL CHEMISTRY A, 2006, 110 (18) :5998-6009
[5]   RETRACTED: Cysteine and glutathione secretion in response to protein disulfide bond formation in the ER (Retracted Article) [J].
Carelli, S ;
Ceriotti, A ;
Cabibbo, A ;
Fassina, G ;
Ruvo, M ;
Sitia, R .
SCIENCE, 1997, 277 (5332) :1681-1684
[6]   A thiol-specific fluorescent probe and its application for bioimaging [J].
Chen, Xiaoqiang ;
Ko, Sung-Kyun ;
Kim, Min Jung ;
Shin, Injae ;
Yoon, Juyoung .
CHEMICAL COMMUNICATIONS, 2010, 46 (16) :2751-2753
[7]   New Chemodosimetric Reagents as Ratiometric Probes for Cysteine and Homocysteine and Possible Detection in Living Cells and in Blood Plasma [J].
Das, Priyadip ;
Mandal, Amal Kumar ;
Chandar, Nellore Bhanu ;
Baidya, Mithu ;
Bhatt, Harshad B. ;
Ganguly, Bishwajit ;
Ghosh, Sudip K. ;
Das, Amitava .
CHEMISTRY-A EUROPEAN JOURNAL, 2012, 18 (48) :15382-15393
[8]   Conversion of cysteine to formylglycine: A protein modification in the endoplasmic reticulum [J].
Dierks, T ;
Schmidt, B ;
VonFigura, K .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (22) :11963-11968
[9]   A BODIPY aldoxime-based chemodosimeter for highly selective and rapid detection of hypochlorous acid [J].
Emrullahoglu, Mustafa ;
Ucuncu, Muhammed ;
Karakus, Erman .
CHEMICAL COMMUNICATIONS, 2013, 49 (71) :7836-7838
[10]   Determination of biological thiols by high-performance liquid chromatography following derivatization by ThioGlo maleimide reagents [J].
Ercal, N ;
Yang, P ;
Aykin, N .
JOURNAL OF CHROMATOGRAPHY B, 2001, 753 (02) :287-292