Regulation of OsSPX1 and OsSPX3 on Expression of OsSPX domain Genes and Pi-starvation Signaling in Rice

被引：112

作者：

Wang, Zhiye ^{[1
]}

Hu, Han ^{[1
]}

Huang, Hongjie ^{[1
]}

Duan, Ke ^{[1
]}

Wu, Zhongchang ^{[1
]}

Wu, Ping ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Life Sci, State Key Lab Plant Physiol & Biochem, Hangzhou 310058, Zhejiang, Peoples R China

来源：

JOURNAL OF INTEGRATIVE PLANT BIOLOGY | 2009年 / 51卷 / 07期

关键词：

Oryza sativa; SPX domain gene; expression regulation; Pi-signaling; MULTIPLE SEQUENCE ALIGNMENT; PHOSPHATE; MICRORNA399; PHO2; DEFICIENCY;

D O I：

10.1111/j.1744-7909.2009.00834.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The rice (Oryza sativa L.) genome contains at least six genes exclusively with an SPX (SYG1/PHO81/XPR1) domain at the N-terminal, designated as OsSPX1-6. Here we report the diverse expression patterns of the OsSPX genes in different tissues and their responses to Pi-starvation. Among them, five genes, OsSPX1, 2, 3, 5 and 6 are responsive to Pi-starvation in shoots and/or in roots. The subcellular localization analysis indicates that OsSPX1 and OsSPX2 is exclusively located in nucleus, OsSPX3 in the cytoplasm, and OsSPX4 is a membrane localization protein. OsSPX1 regulates OsSPX2, 3 and 5 at the transcription level and is positively involved in the responses of the genes to Pi-starvation. Overexpression of OsSPX3 downregulates OsSPX5 in shoots under Pi-sufficiency. OsSPX3 negatively regulates the PSI (Pi-starvation induced) gene, OsIPS1 and is involved in the responses of miR399 and OsPHO2 to Pi-starvation. Our results suggest that OsSPX1 may be a regulator involved in the transcriptions of OsSPX2, 3 and 5. OsSPX3 plays a role in OsIPS1/miR399 mediated long distance regulation on OsPHO2. Our results also indicate that OsSPX3 is involved in plant tolerance to Pi-starvation stress.

引用

页码：663 / 674

页数：12

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