Blunting p38 MAPKa and ERK1/2 activities by empagliflozin enhances the antifibrotic effect of metformin and augments its AMPK-induced NF-κB inactivation in mice intoxicated with carbon tetrachloride

被引:33
作者
Abdelhamid, Amir Mohamed [1 ]
Youssef, Mahmoud E. [1 ]
Abd El-Fattah, Eslam E. [2 ]
Gobba, Naglaa A. [3 ]
Gaafar, Ahmed Gaafar Ahmed [4 ]
Girgis, Samuel [5 ]
Shata, Ahmed [6 ,7 ]
Hafez, Abdel-Moneim [8 ,9 ]
El-Ahwany, Eman [10 ]
Amin, Noha A. [11 ]
Shahien, Mohamed Awad [12 ]
Abd-Eldayem, Marwa A. [13 ]
Abou-Elrous, Magdy [14 ]
Saber, Sameh [1 ]
机构
[1] Delta Univ Sci & Technol, Fac Pharm, Dept Pharmacol, Costal Int Rd,POB 11152, Gamasa, Egypt
[2] Delta Univ Sci & Technol, Fac Pharm, Dept Biochem, Gamasa, Egypt
[3] Misr Univ Sci & Technol, Coll Pharm, Dept Pharmacol & Toxicol, 6th Of October City, Egypt
[4] Port Said Univ, Fac Pharm, Dept Pharmacol & Toxicol, Port Said, Egypt
[5] Alsalam Univ, Fac Pharm, Dept Pharmaceut, Arish, Egypt
[6] Mansoura Univ, Fac Med, Dept Clin Pharmacol, Mansoura, Egypt
[7] Delta Univ Sci & Technol, Fac Pharm, Dept Clin Pharm, Gamasa, Egypt
[8] Mansoura Univ, Fac Med, Dept Med Physiol, Mansoura, Egypt
[9] Qassim Univ, Coll Med, Dept Physiol, Buraydah, Saudi Arabia
[10] Theodor Bilharz Res Inst, Dept Immunol, Giza, Egypt
[11] Theodor Bilharz Res Inst, Dept Hematol, Giza, Egypt
[12] Damietta Univ, Fac Med, Dept Clin Pharmacol, Dumyat, Egypt
[13] Horus Univ, Fac Pharm, Dept Pharmacol & Biochem, New Damietta, Egypt
[14] Helwan Univ, Fac Pharm, Dept Biochem, Cairo, Egypt
关键词
Metformin/empagliflozin; AMPKa; NF-kappa B; p38; MAPK; ERK1/2; Liver fibrosis; ACTIVATED PROTEIN-KINASE; EXTRACELLULAR-MATRIX; HEPATIC-FIBROSIS; LIVER FIBROSIS; INFLAMMATION; INJURY; COMBINATION; DYSFUNCTION; MECHANISMS; COLLAGEN;
D O I
10.1016/j.lfs.2021.120070
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Aim: Metformin and empagliflozin combined therapy may have complementary effects that go beyond the wellrecognized targets of their monotherapy through AMPK activation. Therefore, the current study was designed to investigate for the first time the hepatoprotective effects of such combination therapy in the carbon tetrachloride (CCl4)-induced hepatic fibrosis model in mice. Materials and methods: Determination of liver enzymes and the liver content of oxidative stress parameters, and hydroxyproline were performed biochemically. ELISA was performed to measure PDGF-BB, TNF-alpha, TGF-beta, TIMP1, AMPK, p-mTOR, NF-kappa B P65 binding activity, p38 MAPKa, JNK1/2 and ERK1/2. Real-time qPCR was conducted to determine Col1a1 and a-SMA. In addition, histopathological examination using H&E and Masson's trichrome stain were performed for determination of histopathological changes. Key findings: Empagliflozin inhibited the activation of p38 MAPK and ERK1/2 and exhibited a weak AMPKa stimulation. On the other hand, metformin exerted a more robust stimulatory action on the AMPKa that was accompanied by a notable decrease in the NF-kappa B nuclear binding activity and a decline in the p-mTOR levels. Nevertheless, the effect of metformin on MAPK kinases was insignificant. Our results revealed that blunting p38 MAPKa and ERK1/2 activities by empagliflozin enhanced the antifibrotic effect of metformin and augmented its AMPK-induced NF-kappa B inactivation. Significance: As diabetes is one of the most common risk factors for liver fibrosis, the use of antidiabetic drugs is expected to improve therapeutic outcome. Therefore, metformin/empagliflozin combined therapy could be promising in preventing hepatic inflammation and fibrosis via exhibiting complementary effects particularly in diabetic patients.
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页数:13
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