Development of a minor groove binder assay for real-time PCR detection of porcine Sapelovirus

被引：11

作者：

Chen, Junwei ^{[1
]}

Chen, Feng ^{[2
]}

Zhou, Qingfeng ^{[3
]}

Li, Wei ^{[3
]}

Chen, Yanshan ^{[3
]}

Song, Yanhua ^{[3
]}

Zhang, Xiangbin ^{[2
]}

Xue, Chunyi ^{[1
]}

Bi, Yingzuo ^{[2
]}

Cao, Yongchang ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510006, Guangdong, Peoples R China

[2] South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China

[3] Guangdong Wens Grp Acad, Guangdong Wens Foodstuffs Grp Co Ltd, Xinxing, Guangdong, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2014年 / 198卷

关键词：

Porcine Sapelovirus; Real-time PCR; Minor groove binder probe; Detection; COMPLETE GENOME SEQUENCE; FECAL SAMPLES; TESCHOVIRUSES; PIGLETS;

D O I：

10.1016/j.jviromet.2013.12.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A 5' conjugated minor groove binder (MGB) probe real-time PCR assay was developed in this study for porcine sapelovirus (PSV) detection and quantitation. Two primers and a MGB probe for the 5' untranslated region (UTR) gene were designed. The assay was capable of detecting about 103 copies/mu l of standard template per reaction. Moreover, it does not detect any of the other RNA viruses that cause diarrhea disease in pigs. The coefficients of variation of intra- and inter-assay reproducibility were both lower than 2%. In 73 field fecal samples, PSV was detected in 46 samples using real-time PCR assay and only 32 samples with a conventional PCR assay. Therefore, the availability of this assay will facilitate further studies on the epidemiology of PSV infection and its role in swine disease. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：69 / 74

页数：6

共 22 条

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