PCR-Based detection of Bacillus anthracis in formalin-fixed tissue from a patient receiving ciprofloxacin

被引：11

作者：

Levine, SM

Perez-Perez, G

Olivares, A

Yee, H

Hanna, BA

Blaser, MJ

机构：

[1] NYU, Sch Med, Dept Med, New York, NY 10016 USA

[2] NYU, Sch Med, Dept Microbiol, New York, NY 10016 USA

[3] NYU, Sch Med, Dept Pathol, New York, NY 10016 USA

[4] Vet Affairs Med Ctr, New York, NY USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 11期

关键词：

D O I：

10.1128/JCM.40.11.4360-4362.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We demonstrate that Bacillus anthracis may be detected from a formalin-fixed, paraffin-embedded biopsy specimen, even after the patient has received antibiotic treatment. Although traditional PCR methods may not be sufficiently sensitive for anthrax detection in such patients, cycle numbers can be increased or PCR can be repeated by using an aliquot from a previous PCR as the template.

引用

收藏

页码：4360 / 4362

页数：3

相关论文

共 19 条

[1] The native virulence plasmid combination affects the segregational stability of a theta-replicating shuttle vector in Bacillus anthracis var. New Hampshire [J].

Bowen, JE ;

Quinn, CP .

JOURNAL OF APPLIED MICROBIOLOGY, 1999, 87 (02) :270-278

[2] Index case of fatal inhalational anthrax due to bioterrorism in the United States [J].

Bush, LM ;

Abrams, BH ;

Beall, A ;

Johnson, CC .

NEW ENGLAND JOURNAL OF MEDICINE, 2001, 345 (22) :1607-1610

[3]

*CDCP, 2001, MMWR-MORBID MORTAL W, V50, P1008

[4] Differential influence of the two Bacillus anthracis plasmids on regulation of virulence gene expression [J].

Fouet, A ;

Mock, M .

INFECTION AND IMMUNITY, 1996, 64 (12) :4928-4932

[5] Cutaneous Bacillus anthracis infection [J].

Gallagher, TC ;

Strober, BE .

NEW ENGLAND JOURNAL OF MEDICINE, 2001, 345 (22) :1646-1647

[6] DEMONSTRATION OF A CAPSULE PLASMID IN BACILLUS-ANTHRACIS [J].

GREEN, BD ;

BATTISTI, L ;

KOEHLER, TM ;

THORNE, CB ;

IVINS, BE .

INFECTION AND IMMUNITY, 1985, 49 (02) :291-297

[7]

INGLESBY TV, 1999, JAMA-J AM MED ASSOC, V281, P1635

[8] PCR analysis of tissue samples from the 1979 Sverdlovsk anthrax victims:: The presence of multiple Bacillus anthracis strains in different victims [J].

Jackson, PJ ;

Hugh-Jones, ME ;

Adair, DM ;

Green, G ;

Hill, KK ;

Kuske, CR ;

Grinberg, LM ;

Abramova, FA ;

Keim, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (03) :1224-1229

[9] Bioterrorism-related inhalational anthrax: The first 10 cases reported in the United States [J].

Jernigan, JA ;

Stephens, DS ;

Ashford, DA ;

Omenaca, C ;

Topiel, MS ;

Galbraith, M ;

Tapper, M ;

Fisk, TL ;

Zaki, S ;

Popovic, T ;

Meyer, RF ;

Quinn, CP ;

Harper, SA ;

Fridkin, SK ;

Sejvar, JJ ;

Shepard, CW ;

McConnell, M ;

Guarner, J ;

Shieh, WJ ;

Malecki, JM ;

Gerberding, JL ;

Hughes, JM ;

Perkins, BA .

EMERGING INFECTIOUS DISEASES, 2001, 7 (06) :933-944

[10] EVIDENCE FOR PLASMID-MEDIATED TOXIN PRODUCTION IN BACILLUS-ANTHRACIS [J].

MIKESELL, P ;

IVINS, BE ;

RISTROPH, JD ;

DREIER, TM .

INFECTION AND IMMUNITY, 1983, 39 (01) :371-376