An in vitro system from Plasmodium falciparum active in endogenous mRNA translation

被引：6

作者：

Ferreras, A ^{[1
]}

Triana, L ^{[1
]}

Correia, H ^{[1
]}

Sánchez, E ^{[1
]}

Herrera, F ^{[1
]}

机构：

[1] Univ Carabobo Nucl Aragua, Fac Ciencias Salud, Ctr Invest Biomed, Maracay, Edo Aragua, Venezuela

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2000年 / 95卷 / 02期

关键词：

Plasmodium falciparum; protein synthesis; cell free translation; parasite lysate;

D O I：

10.1590/S0074-02762000000200017

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

An in vitro translation system has been prepared from Plasmodium falciparum by saponin lysis of infected-erythrocytes to free parasites which were homogeneized with glass beads, centrifuged to obtain a S-30 fraction followed by Sephadex G-25 gel filtration. This treatment produced a system with very low contamination of host proteins (<1%). The system, optimized for Mg2+ and K+, translates endogenous mRNA and is active for 80 min which suggests that their protein factors and mRNA are quite stable.

引用

页码：231 / 235

页数：5

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