Mapping the distribution of 3-hydroxy oxylipins in the ascomycetous yeast Saturnispora saitoi

被引：2
|
作者：
Bareetseng, Andries S.
Kock, Johan L. F.
Pohl, Carolina H.
Pretorius, Elma E.
Strauss, Catharina J.
Botes, Piet J.
Van Wyk, Pieter W. J.
Nigam, Santosh
机构：
[1] Univ Free State, Dept Microbial Biochem & Food Biotechnol, UNESCO, MIRCEN Ind Biotechnol, Bloemfontein, South Africa
[2] Univ Free State, Ctr Confocal & Electron Microscopy, Bloemfontein, South Africa
[3] Free Univ Berlin, Univ Med Ctr Benjamin Franklin, Dept Gynaecol, Eicosanoid Res Div, D-12200 Berlin, Germany
来源：
SYSTEMATIC AND APPLIED MICROBIOLOGY | 2006年 / 29卷 / 06期
基金：
新加坡国家研究基金会;
关键词：
ascospores; electron microscopy; 3-hydroxy oxylipins; immunofluorescence; Saturnispora saitoi; ultrastructure; yeast;
D O I：
10.1016/j.syapm.2005.11.008
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The distribution of 3-hydroxy oxylipins in Saturnispora saitoi was mapped using immunofluorescence microscopy. Fluorescence was observed on aggregating ascospores, indicating the presence of 3-hydroxy oxylipins on the surface or between ascospores. The oxylipin was identified as 3-hydroxy 9:1 using gas chromatography mass spectrometry. Furthermore, ultrastructural studies using scanning and transmission electron microscopy on ascospores revealed a clear equatorial ledge surrounding oval-shaped ascospores. (c) 2005 Elsevier GmbH. All rights reserved.
引用
收藏
页码：446 / 449
页数：4
相关论文
共 22 条
123