α-amanitin resistance in Drosophila melanogaster: A genome-wide association approach

被引：8

作者：

Mitchell, Chelsea L. ^{[1
]}

Latuszek, Catrina E. ^{[1
]}

Vogel, Kara R. ^{[2
]}

Greenlund, Ian M. ^{[1
]}

Hobmeier, Rebecca E. ^{[1
]}

Ingram, Olivia K. ^{[1
]}

Dufek, Shannon R. ^{[1
]}

Pecore, Jared L. ^{[1
]}

Nip, Felicia R. ^{[3
]}

Johnson, Zachary J. ^{[4
]}

Ji, Xiaohui ^{[5
]}

Wei, Hairong ^{[5
]}

Gailing, Oliver ^{[5
]}

Werner, Thomas ^{[1
]}

机构：

[1] Michigan Technol Univ, Dept Biol Sci, 1400 Townsend Dr, Houghton, MI 49931 USA

[2] Univ Wisconsin, Dept Neurol, Sch Med & Publ Hlth, 1300 Univ Ave, Madison, WI 53706 USA

[3] Michigan State Univ, Coll Human Med, Ctr Clin, E Lansing, MI 48824 USA

[4] US Forest Serv, Salt Lake Ranger Dist 6944 S,3000 E, Salt Lake City, UT USA

[5] Michigan Technol Univ, Sch Forest Resources & Environm Sci, 1400 Townsend Dr, Houghton, MI 49931 USA

来源：

PLOS ONE | 2017年 / 12卷 / 02期

关键词：

GENETIC REFERENCE PANEL; RNA POLYMERASE-II; INSECTICIDE RESISTANCE; MYCOPHAGOUS DROSOPHILA; EMBRYONIC MIDGUT; INNATE IMMUNITY; LIFE-SPAN; POPULATION; EXPRESSION; AUTOPHAGY;

D O I：

10.1371/journal.pone.0173162

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We investigated the mechanisms of mushroom toxin resistance in the Drosophila Genetic Reference Panel (DGRP) fly lines, using genome-wide association studies (GWAS). While Drosophila melanogaster avoids mushrooms in nature, some lines are surprisingly resistant to alpha-amanitin-a toxin found solely in mushrooms. This resistance may represent a pre-adaptation, which might enable this species to invade the mushroom niche in the future. Although our previous microarray study had strongly suggested that pesticide-metabolizing detoxification genes confer alpha-amanitin resistance in a Taiwanese D. melanogaster line Ama-KTT, none of the traditional detoxification genes were among the top candidate genes resulting from the GWAS in the current study. Instead, we identified Megalin, Tequila, and widerborst as candidate genes underlying the alpha-amanitin resistance phenotype in the North American DGRP lines, all three of which are connected to the Target of Rapamycin (TOR) pathway. Both widerborst and Tequila are upstream regulators of TOR, and TOR is a key regulator of autophagy and Megalin-mediated endocytosis. We suggest that endocytosis and autophagy of alpha-amanitin, followed by lysosomal degradation of the toxin, is one of the mechanisms that confer alpha-amanitin resistance in the DGRP lines.

引用

页数：16