Peroxidase-like activity of magnetoferritin

被引：31

作者：

Melnikova, Lucia ^{[1
]}

Pospiskova, Kristyna ^{[2
,4
]}

Mitroova, Zuzana ^{[1
]}

Kopcansky, Peter ^{[1
]}

Safarik, Ivo ^{[3
,4
]}

机构：

[1] Slovakian Acad Sci, Inst Expt Phys, Kosice 04001, Slovakia

[2] Palacky Univ, Fac Sci, Dept Biochem, Olomouc 78371, Czech Republic

[3] GCRC, Inst Nanobiol & Struct Biol, Dept Nanobiotechnol, Ceske Budejovice 37005, Czech Republic

[4] Palacky Univ, Reg Ctr Adv Technol & Mat, Olomouc 78371, Czech Republic

来源：

MICROCHIMICA ACTA | 2014年 / 181卷 / 3-4期

关键词：

Magnetoferritin; Magnetic nanoparticles; Peroxidase-like activity; Hydrogen peroxide; Oxidative stress; HYDROGEN-PEROXIDE; MAGNETIC NANOPARTICLES; ALZHEIMERS-DISEASE; OXIDATIVE STRESS; IRON; FERRITIN; AGGREGATION; RADICALS; METALS; BETA;

D O I：

10.1007/s00604-013-1105-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Magnetoferritin is a spherical biomacromolecule with a diameter of about 12 nm. It consists of a protein shell composed of apoferritin that is surrounding magnetic nanoparticles of magnetite (Fe3O4) or maghemite (gamma-Fe2O3). Magnetoferritins with various iron content (loading factor) were synthetically prepared and their peroxidase-like activities studied via the oxidation of the chromogenic substrate N,N-diethyl-p-phenylenediamine sulfate by hydrogen peroxide to give a purple product with an absorption maximum at 551 nm. Magnetoferritin with higher loading factor exhibits a higher peroxidase-like activity. The catalytic activity was successfully applied to the determination of hydrogen peroxide in the 5.8 to 88.2 mM concentration range.

引用

页码：295 / 301

页数：7

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