Phosphate transporter mediated lipid accumulation in Saccharomyces cerevisiae under phosphate starvation conditions

被引：13
|
作者：
James, Antoni W. [1 ]
Nachiappan, Vasanthi [1 ]
机构：
[1] Bharathidasan Univ, Sch Life Sci, Biomembrane Lab, Dept Biochem, Tiruchirappalli 620024, Tamil Nadu, India
来源：
BIORESOURCE TECHNOLOGY | 2014年 / 151卷
关键词：
Biodiesel; Saccharomyces cerevisiae; Triacylglycerol; Acyltransferase; Phosphate transporter; BIODIESEL PRODUCTION;
D O I：
10.1016/j.biortech.2013.10.054
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
In the current study, when phosphate transporters pho88 and pho86 were knocked out they resulted in significant accumulation (84% and 43%) of triacylglycerol (TAG) during phosphate starvation. However in the presence of phosphate, TAG accumulation was only around 45% in both pho88 and pho86 mutant cells. These observations were confirmed by radio-labeling, fluorescent microscope and RT-PCR studies. The TAG synthesizing genes encoding for acyltransferases namely LRO1 and DGA1 were up regulated. This is the first report for accumulation of TAG in pho88 Delta and pho86 Delta cells under phosphate starvation conditions. (C) 2013 Published by Elsevier Ltd.
引用
收藏
页码：100 / 105
页数：6
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