Long-term in vitro and in vivo effects of γ-irradiated BCG on innate and adaptive immunity

被引:73
作者
Arts, Rob J. W. [1 ]
Blok, Bastiaan A. [1 ,3 ,4 ]
Aaby, Peter [3 ]
Joosten, Leo A. B. [1 ]
de Jong, Dirk [2 ]
van der Meer, Jos W. M. [1 ]
Benn, Christine Stabell [3 ,4 ]
van Crevel, Reinout [1 ]
Netea, Mihai G. [1 ]
机构
[1] Radboud Univ Nijmegen, Med Ctr, Dept Internal Med, NL-6525 GA Nijmegen, Netherlands
[2] Radboud Univ Nijmegen, Med Ctr, Dept Gastroenterol, NL-6525 GA Nijmegen, Netherlands
[3] Statens Serum Inst, Bandim Hlth Project, Res Ctr Vitamins & Vaccines, DK-2300 Copenhagen, Denmark
[4] Univ Southern Denmark, Odense Univ Hosp, Odense Patient Data Explorat Network, Copenhagen, Denmark
基金
新加坡国家研究基金会;
关键词
trained immunity; heterologous immunity; nonspecific vaccination effects; BACILLUS-CALMETTE-GUERIN; CD8(+) T-CELLS; IFN-GAMMA; LISTERIA-MONOCYTOGENES; BYSTANDER ACTIVATION; RANDOMIZED-TRIAL; VACCINATION; TUBERCULOSIS; RESPONSES; MEMORY;
D O I
10.1189/jlb.4MA0215-059R
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
BCG vaccination is associated with a reduced mortality from nonmycobacterial infections. This is likely to be mediated by a combination of innate-immune memory ("trained immunity") and heterologous effects on adaptive immunity. As such, BCG could be used to boost host immunity but not in immunocompromised hosts, as it is a live, attenuated vaccine. Therefore, we assessed whether killed gamma BCG has similar potentiating effects. In an in vitro model of trained immunity, human monocytes were incubated with gamma BCG for 24 h and restimulated after 6 d. Cytokine production and the role of pattern recognition receptors and histone methylation markers were assessed. The in vivo effects of gamma BCG vaccination were studied in a proof-of-principle trial in 15 healthy volunteers. gamma BCG induced trained immunity in vitro via the NOD2 receptor pathway and up-regulation of H3K4me3 histone methylation. However, these effects were less strong than those induced by live BCG. gamma BCG vaccination in volunteers had only minimal effects on innate immunity, whereas a significant increase in heterologous Th1/Th17 immunity was observed. Our results indicate that gamma BCG induces long-term training of innate immunity in vitro. In vivo, gamma BCG induces mainly heterologous effects on the adaptive-immune system, whereas effects on innate cytokine production are limited.
引用
收藏
页码:995 / 1001
页数:7
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