Physiological and genetic characterization of rice nitrogen fixer PGPR isolated from rhizosphere soils of different crops

被引：73

作者：

Habibi, Safiullah ^{[1
]}

Djedidi, Salem ^{[2
]}

Prongjunthuek, Kunlayakorn ^{[3
]}

Mortuza, Md Firoz ^{[4
]}

Ohkama-Ohtsu, Naoko ^{[5
]}

Sekimoto, Hitoshi ^{[6
]}

Yokoyoma, Tadashi ^{[5
]}

机构：

[1] Tokyo Univ Agr & Technol, United Grad Sch Agr, Tokyo, Japan

[2] Tokyo Univ Agr & Technol, Fac Agr, Tokyo, Japan

[3] Dept Agr, Soil Sci Div, Soil Microbiol Res Grp, Bangkok, Thailand

[4] Bangladesh Atom Energy Commiss, Inst Food & Radiat Biol, Dhaka, Bangladesh

[5] Tokyo Univ Agr & Technol, Inst Agr, Tokyo, Japan

[6] Utsunomiya Univ, Fac Agr, Utsunomiya, Tochigi 321, Japan

来源：

PLANT AND SOIL | 2014年 / 379卷 / 1-2期

基金：

日本学术振兴会;

关键词：

Rhizosphere; Plant growth promoting rhizobacteria; Rice; Nitrogen fixation; IAA; Phosphate solubilization; 16S rRNA; Bacillus altitudinis; Pseudomonas monteilii; Pseudomonas mandelii; Rhizobium daejeonense; GROWTH-PROMOTING BACTERIA; PLANT-GROWTH; MICROBIAL DIVERSITY; COMMUNITY; FIELDS; MAIZE; FIXATION; MODEL; GRASS; WHEAT;

D O I：

10.1007/s11104-014-2035-7

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

We aimed to identify plant growth-promoting rhizobacteria that could be used to develop a biofertilizer for rice. To obtain plant growth-promoting rhizobacteria, rhizosphere soils from different crops (rice, wheat, oats, crabgrass, maize, ryegrass, and sweet potato) were inoculated to rice plants. In total, 166 different bacteria were isolated and their plant growth-promoting traits were evaluated in terms of colony morphology, indole-3-acetic acid production, acetylene reduction activity, and phosphate solubilization activity. Moreover, genetic analysis was carried out to evaluate their phylogenetic relationships based on 16S rRNA sequence data. Strains of Bacillus altitudinis, Pseudomonas monteilii, and Pseudomonas mandelii formed associations with rice plants and fixed nitrogen. A strain of Rhizobium daejeonense showed nitrogen fixation activity in an in vitro assay and in vivo. Strains of B. altitudinis and R. daejeonense derived from rice rhizosphere soil, strains of P. monteilii and Enterobacter cloacae derived from wheat rhizosphere soil, and a strain of Bacillus pumilus derived from maize rhizosphere soil significantly promoted rice plant growth. These methods are effective to identify candidate species that could be developed as biofertilizers for target crops.

引用

页码：51 / 66

页数：16

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