FATTY ACIDS DETERMINATION IN TROUT PLASMA AND MEAT BY GC-MS

被引:0
|
作者
Suvar, Sonia [1 ]
Horj, Elena [1 ]
Podea, Paula [2 ]
Iordache, Andreea [3 ]
Cocan, Daniel [4 ]
Culea, Monica [1 ]
机构
[1] Univ Babes Bolyai, Fac Phys, 1 Kogalniceanu Str, Cluj Napoca 400084, Romania
[2] Univ Babes Bolyai, Fac Chem & Chem Engn, RO-400084 Cluj Napoca, Romania
[3] Natl R&D Inst Cryogen & Isotop Technol, Ramnicu Valcea 240050, Romania
[4] Univ Agr Sci & Vet Med, Cluj Napoca 400372, Romania
来源
关键词
DHA; EPA; essential fatty acids; FAME; GC-MS; SFA; UFA; MASS-SPECTROMETRY; FISH; WILD;
D O I
暂无
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The aim of this study was to develop a simple and reliable GC-MS method to compare the fatty acids in trout plasma and meat. The lipids were extracted from 0.5 mL of plasma and 1 g of meat using chloroform:methanol 1:2 (v:v) and then derivatized into fatty acids methyl esters (FAMEs) by esterification with methanol:acetyl chloride 4:1 (v:v). For FAMEs quantitation, undecaenoic acid (C11:1) was used as internal standard. High proportions of unsaturated fatty acids (UFAs) were found both in plasma and in meat samples. The highest proportion of UFAs in meat samples (53%) was represented by the omega-3 fatty acids.
引用
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页码:109 / 116
页数:8
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