The Cellular Senescence Factor Extracellular HMGB1 Directly Inhibits Oligodendrocyte Progenitor Cell Differentiation and Impairs CNS Remyelination

被引:14
|
作者
Rouillard, Megan E. [1 ]
Hu, Jingwen [2 ,3 ]
Sutter, Pearl A. [1 ]
Kim, Hee Won [2 ,3 ]
Huang, Jeffrey K. [2 ,3 ]
Crocker, Stephen J. [1 ]
机构
[1] Univ Connecticut, Dept Neurosci, Sch Med, Farmington, CT USA
[2] Georgetown Univ, Dept Biol, Washington, DC USA
[3] Georgetown Univ, Ctr Cell Reprogramming, Washington, DC USA
关键词
cellular senescence; myelin; multiple sclerosis; lysolecithin (LPC); microglia; TLR2; spinal cord; MOBILITY GROUP BOX-1; MULTIPLE-SCLEROSIS LESIONS; BLOOD-BRAIN-BARRIER; NF-KAPPA-B; PROINFLAMMATORY CYTOKINE; PRECURSOR CELLS; PROTEIN HMGB1; ACTIVATION; RELEASE; DEMYELINATION;
D O I
10.3389/fncel.2022.833186
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
HMGB1 is a highly conserved, ubiquitous protein in eukaryotic cells. HMGB1 is normally localized to the nucleus, where it acts as a chromatin associated non-histone binding protein. In contrast, extracellular HMGB1 is an alarmin released by stressed cells to act as a danger associated molecular pattern (DAMP). We have recently determined that progenitor cells from multiple sclerosis patients exhibit a cellular senescent phenotype and release extracellular HMGB1 which directly impaired the maturation of oligodendrocyte progenitor cells (OPCs) to myelinating oligodendrocytes (OLs). Herein, we report that administration of recombinant HMGB1 into the spinal cord at the time of lysolecithin administration resulted in arrest of OPC differentiation in vivo, and a profound impairment of remyelination. To define the receptor by which extracellular HMGB1 mediates its inhibitory influence on OPCs to impair OL differentiation, we tested selective inhibitors against the four primary receptors known to mediate the effects of HMGB1, the toll-like receptors (TLRs)-2, -4, -9 or the receptor for advanced glycation end-products (RAGE). We found that inhibition of neither TLR9 nor RAGE increased OL differentiation in the presence of HMGB1, while inhibition of TLR4 resulted in partial restoration of OL differentiation and inhibiting TLR2 fully restored differentiation of OLs in the presence of HMGB1. Analysis of transcriptomic data (RNAseq) from OPCs identified an overrepresentation of NF kappa B regulated genes in OPCs when in the presence of HMGB1. We found that application of HMGB1 to OPCs in culture resulted in a rapid and concentration dependent shift in NF kappa B nuclear translocation which was also attenuated with coincident TLR2 inhibition. These data provide new information on how extracellular HMGB1 directly affects the differentiation potential of OPCs. Recent and past evidence for elevated HMGB1 released from senescent progenitor cells within demyelinated lesions in the MS brain suggests that a greater understanding of how this molecule acts on OPCs may unfetter the endogenous remyelination potential in MS.
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页数:14
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