Directed evolution of cyclohexanone monooxygenases: Enantioselective biocatalysts for the oxidation of prochiral thioethers

被引：88
作者：
Reetz, MT [1 ]
Daligault, F [1 ]
Brunner, B [1 ]
Hinrichs, H [1 ]
Deege, A [1 ]
机构：
[1] Max Planck Inst Kohlenforsch, D-45470 Mulheim, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 31期
关键词：
asymmetric catalysis; directed evolution; enzyme catalysis; protein engineering; sulfoxides;
D O I：
10.1002/anie.200460311
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Mutational changes in cyclohexanone monooxygenases (CHMOs), for example the introduction of serine at position 432, lead to surprisingly versatile enantioselective biocatalysts that perform particularly well in the air-mediated partial oxidation of prochiral thioethers (see scheme).
引用
收藏
页码：4078 / 4081
页数：4
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