Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging

被引：40

作者：

He, Ruei-Yu ^{[2
]}

Su, Yuan-Deng ^{[1
]}

Cho, Keng-Chi ^{[3
]}

Lin, Chun-Yun ^{[1
]}

Chang, Nan-Shan ^{[4
]}

Chang, Chih-Han ^{[2
]}

Chen, Shean-Jen ^{[1
]}

机构：

[1] Natl Cheng Kung Univ, Dept Engn Sci, Tainan 701, Taiwan

[2] Natl Cheng Kung Univ, Inst Biomed Engn, Tainan 701, Taiwan

[3] Natl Cheng Kung Univ, Inst Electroopt Sci & Engn, Tainan 701, Taiwan

[4] Natl Cheng Kung Univ, Inst Mol Med, Tainan 701, Taiwan

来源：

OPTICS EXPRESS | 2009年 / 17卷 / 08期

关键词：

TOTAL INTERNAL-REFLECTION; DOMAIN-CONTAINING OXIDOREDUCTASE; EVANESCENT-FIELD; GOLD NANOPARTICLES; COUPLED EMISSION; EXCITATION; METAL; SPECTROSCOPY; ABSORPTION; SCATTERING;

D O I：

10.1364/OE.17.005987

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide fluorescent images of living cell membranes. The proposed microscope with the help of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having a shorter fluorophore lifetime. In comparison with a one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. By combining the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated it's capability for brighter and more contrasted fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct. (C) 2009 Optical Society of America

引用

页码：5987 / 5997

页数：11

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