Direct binding procedure of proteins and enzymes to fine magnetic particles

被引：77
作者：
Koneracká, M
Kopcansky, P
Timko, M
Ramchand, CN
de Sequeira, A
Trevan, M
机构：
[1] Slovak Acad Sci, Inst Expt Phys, Kosice 04353, Slovakia
[2] Univ Sheffield, Sheffield S5 7AU, S Yorkshire, England
[3] Bath Spa Univ Coll, Sch Sci & Environm, Bath BA2 9BN, Avon, England
[4] Univ Westminster, Sch Biosci, London W1M 8JS, England
来源：
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2002年 / 18卷 / 1-3期
关键词：
immobilisation; magnetic particles; proteins; enzymes; biotechnology; biomedicine;
D O I：
10.1016/S1381-1177(02)00016-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Several clinically important proteins and enzymes (bovine serum albumin (BSA), glucose oxidase (GOD) (EC 1.1.3.4), streptokinase (EC 3.4.99.0), chymotrypsin (EC 3.4.21.1) and dispase (EC 3.4.24.3)), respectively, have been immobilised onto fine magnetic particles using carbodiimide as a coupling agent. The coupling reactions of these substances were carried out using various ratios of magnetic particles to protein, and different values of pH to determine the optimum conditions of immobilisation. The possible applications in biomedicine and biotechnology of this method of immobilisation are discussed. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：13 / 18
页数：6
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