Cryo-Electron Tomography Studies of Cell Systems

被引：1
|
作者：
Kamyshinsky, R. A. [1 ,2 ]
Chesnokov, Y. M. [1 ,2 ]
Orekhov, A. S. [1 ,2 ]
机构：
[1] Kurchatov Inst, Natl Res Ctr, Moscow 123182, Russia
[2] Russian Acad Sci, Shubnikov Inst Crystallog, Fed Res Ctr Crystallog & Photon, Moscow 119333, Russia
来源：
CRYSTALLOGRAPHY REPORTS | 2020年 / 65卷 / 05期
基金：
俄罗斯科学基金会;
关键词：
ELECTRON CRYO-TOMOGRAPHY; MACROMOLECULAR COMPLEXES; VITREOUS SECTIONS; CUTTING ARTIFACTS; EM STRUCTURE; IMPLEMENTATION; RESOLUTION; VISUALIZATION; ARCHITECTURE; PERFORMANCE;
D O I：
10.1134/S1063774520050090
中图分类号：
O7 [晶体学];
学科分类号：
0702 ; 070205 ; 0703 ; 080501 ;
摘要：
Cryo-electron tomography is a powerful tool for determining the three-dimensional structures of macromolecular complexes in their natural environment. The minimization of external impacts on the investigated objects, possibility of conductingin vitroandin celluloexperiments under close-to-native conditions, and high spatial resolution of the obtained three-dimensional reconstructions make cryo-electron tomography one of the most promising methods for studying a large class of objects in the fields of structural biology and visual proteomics. The main aspects of cryo-electron tomography are discussed as applied to studies of cell systems.
引用
收藏
页码：744 / 748
页数：5
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