Cryo-Electron Tomography Studies of Cell Systems

被引：1

作者：

Kamyshinsky, R. A. ^{[1
,2
]}

Chesnokov, Y. M. ^{[1
,2
]}

Orekhov, A. S. ^{[1
,2
]}

机构：

[1] Kurchatov Inst, Natl Res Ctr, Moscow 123182, Russia

[2] Russian Acad Sci, Shubnikov Inst Crystallog, Fed Res Ctr Crystallog & Photon, Moscow 119333, Russia

来源：

CRYSTALLOGRAPHY REPORTS | 2020年 / 65卷 / 05期

基金：

俄罗斯科学基金会;

关键词：

ELECTRON CRYO-TOMOGRAPHY; MACROMOLECULAR COMPLEXES; VITREOUS SECTIONS; CUTTING ARTIFACTS; EM STRUCTURE; IMPLEMENTATION; RESOLUTION; VISUALIZATION; ARCHITECTURE; PERFORMANCE;

D O I：

10.1134/S1063774520050090

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

Cryo-electron tomography is a powerful tool for determining the three-dimensional structures of macromolecular complexes in their natural environment. The minimization of external impacts on the investigated objects, possibility of conductingin vitroandin celluloexperiments under close-to-native conditions, and high spatial resolution of the obtained three-dimensional reconstructions make cryo-electron tomography one of the most promising methods for studying a large class of objects in the fields of structural biology and visual proteomics. The main aspects of cryo-electron tomography are discussed as applied to studies of cell systems.

引用

页码：744 / 748

页数：5

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