Expression of Gallus Epidermal Growth Factor (gEGF) with Food-Grade Lactococcus lactis Expression System and Its Biological Effects on Broiler Chickens

被引：11
|
作者：
Zhou, Yu [1 ,2 ]
Chen, Pinpin [1 ,2 ]
Shi, Shuai [1 ,2 ]
Li, Xiaowen [1 ,2 ]
Shi, Deshi [1 ,2 ]
Zhou, Zutao [1 ,2 ]
Li, Zili [1 ,2 ]
Xiao, Yuncai [1 ,2 ]
机构：
[1] Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China
[2] Huazhong Agr Univ, Coll Vet Med, Key Lab Prevent Vet Med Hubei Prov, Wuhan 430070, Peoples R China
来源：
BIOMOLECULES | 2021年 / 11卷 / 01期
关键词：
gallus epidermal growth factor; Lactococcus lactis; growth performance; immune function; intestinal development; broiler chickens;
D O I：
10.3390/biom11010103
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
As a multifunctional polypeptide, epidermal growth factor (EGF) increases growth performance or enhances resistance to diseases in commercial broilers under adverse conditions. In this study, a recombinant Lactococcus lactis was established to produce the secretory form of bioactive gEGF. The results of in vitro testing showed that gEGF promoted the proliferation of chicken embryo fibroblast cells. A total of 63 5-day-old broiler chickens were evenly divided into three groups and treated with either M17 medium (the control group), supernatant of LL-pNZ8149 fermentation product (the P-LL group), or supernatant of LL-pNZ8149-gEGF fermentation product (the gEGF group). In two weeks, many measurements of growth, immunity and the intestines were significantly higher in the gEGF group than those in the control and the P-LL groups. Our study showed that the bioactive gEGF could be expressed with Lactococcus lactis expression system with the potential to enhance growth performance, immune function, and intestinal development in broiler chickens.
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页码：1 / 15
页数：15
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