Multi-modal label-free imaging based on a femtosecond fiber laser

被引：14

作者：

Xie, Ruxin ^{[1
]}

Su, Jue ^{[1
]}

Rentchler, Eric C. ^{[2
]}

Zhang, Ziyan ^{[3
]}

Johnson, Carey K. ^{[2
]}

Shi, Honglian ^{[3
]}

Hui, Rongqing ^{[1
]}

机构：

[1] Univ Kansas, Dept Elect Engn & Comp Sci, Lawrence, KS 66045 USA

[2] Univ Kansas, Dept Chem, Lawrence, KS 66045 USA

[3] Univ Kansas, Dept Pharmacol & Toxicol, Lawrence, KS 66045 USA

来源：

BIOMEDICAL OPTICS EXPRESS | 2014年 / 5卷 / 07期

基金：

美国国家卫生研究院;

关键词：

STOKES-RAMAN SCATTERING; HIGH-SPECTRAL-RESOLUTION; CARS MICROSCOPY; HIGH-SENSITIVITY; LIVE CELLS; SPECTROSCOPY; FLUORESCENCE; OSCILLATOR; EXCITATION; SOLITON;

D O I：

10.1364/BOE.5.002390

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate multi-mode microscopy based on a single femtosecond fiber laser. Coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS) and photothermal images can be obtained simultaneously with this simplified setup. Distributions of lipid and hemoglobin in sliced mouse brain samples and blood cells are imaged. The dependency of signal amplitude on the pump power and pump modulation frequency is characterized, which allows to isolate the impact from different contributions. (C) 2014 Optical Society of America

引用

页码：2390 / 2396

页数：7

共 50 条

[31] Photoluminescent polymer nanoparticles for label-free cellular imaging [J].

Lee, Kyung Jin ;

Oh, Wan-Kyu ;

Song, Jooyoung ;

Kim, Sojin ;

Lee, Jiwoon ;

Jang, Jyongsik .

CHEMICAL COMMUNICATIONS, 2010, 46 (29) :5229-5231

[32] Label-free Molecular Imaging and Analysis by Raman Spectroscopy [J].

Kumamoto, Yasuaki ;

Harada, Yoshinori ;

Takamatsu, Tetsuro ;

Tanaka, Hideo .

ACTA HISTOCHEMICA ET CYTOCHEMICA, 2018, 51 (03) :101-110

[33] Quadrant darkfield for label-free imaging of intracellular puncta [J].

Moustafa, Tarek E. ;

Belote, Rachel L. ;

Polanco, Edward R. ;

Judson-Torres, Robert L. ;

Zangle, Thomas A. .

JOURNAL OF BIOMEDICAL OPTICS, 2024, 29 (11)

[34] Label-Free Optical Metabolic Imaging in Cells and Tissues [J].

Georgakoudi, Irene ;

Quinn, Kyle P. .

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2023, 25 :413-443

[35] Hyperspectral backscatter imaging: a label-free approach to cytogenetics [J].

Rebner, Karsten ;

Ostertag, Edwin ;

Kessler, Rudolf W. .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2016, 408 (21) :5701-5709

[36] Multimodal imaging of bacterial species for label-free classification [J].

Dyroy, Piet Constantin ;

Heitz, Julius ;

Studier, Hauke ;

Johannsmeier, Sonja ;

Ripken, Tammo .

LABEL-FREE BIOMEDICAL IMAGING AND SENSING, LBIS 2024, 2024, 12854

[37] Label-Free Optical Fiber Glucose Biosensor Based on Two-Mode Interference [J].

Cheng, Tonglei ;

Sun, Yuanqi ;

Yan, Xin ;

Zhang, Xuenan ;

Wang, Fang ;

Zhou, Xue .

IEEE SENSORS JOURNAL, 2023, 23 (11) :11642-11647

[38] A Fiber Bragg Grating Sensor Based on Cladding Mode Resonance for Label-Free Biosensing [J].

Chen, Shimeng ;

Zhang, Chao ;

Wang, Jiahui ;

Li, Na ;

Song, Yongxin ;

Wu, Haojun ;

Liu, Yun .

BIOSENSORS-BASEL, 2023, 13 (01)

[39] Label-free imaging of semiconducting and metallic carbon nanotubes in cells and mice using transient absorption microscopy [J].

Tong, Ling ;

Liu, Yuxiang ;

Dolash, Bridget D. ;

Jung, Yookyung ;

Slipchenko, Mikhail N. ;

Bergstrom, Donald E. ;

Cheng, Ji-Xin .

NATURE NANOTECHNOLOGY, 2012, 7 (01) :56-61

[40] Label-free Chemically Specific Imaging in Planta with Stimulated Raman Scattering Microscopy [J].

Mansfield, Jessica C. ;

Littlejohn, George R. ;

Seymour, Mark P. ;

Lind, Rob J. ;

Perfect, Sarah ;

Moger, Julian .

ANALYTICAL CHEMISTRY, 2013, 85 (10) :5055-5063

← 1 2 3 4 5 →