Multi-modal label-free imaging based on a femtosecond fiber laser

被引：13
|
作者：
Xie, Ruxin [1 ]
Su, Jue [1 ]
Rentchler, Eric C. [2 ]
Zhang, Ziyan [3 ]
Johnson, Carey K. [2 ]
Shi, Honglian [3 ]
Hui, Rongqing [1 ]
机构：
[1] Univ Kansas, Dept Elect Engn & Comp Sci, Lawrence, KS 66045 USA
[2] Univ Kansas, Dept Chem, Lawrence, KS 66045 USA
[3] Univ Kansas, Dept Pharmacol & Toxicol, Lawrence, KS 66045 USA
来源：
BIOMEDICAL OPTICS EXPRESS | 2014年 / 5卷 / 07期
基金：
美国国家卫生研究院;
关键词：
STOKES-RAMAN SCATTERING; HIGH-SPECTRAL-RESOLUTION; CARS MICROSCOPY; HIGH-SENSITIVITY; LIVE CELLS; SPECTROSCOPY; FLUORESCENCE; OSCILLATOR; EXCITATION; SOLITON;
D O I：
10.1364/BOE.5.002390
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We demonstrate multi-mode microscopy based on a single femtosecond fiber laser. Coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS) and photothermal images can be obtained simultaneously with this simplified setup. Distributions of lipid and hemoglobin in sliced mouse brain samples and blood cells are imaged. The dependency of signal amplitude on the pump power and pump modulation frequency is characterized, which allows to isolate the impact from different contributions. (C) 2014 Optical Society of America
引用
收藏
页码：2390 / 2396
页数：7
相关论文
共 50 条
  • [1] Miniature device for chronic, label-free multi-modal optical imaging of cortical hemodynamics in rats
    Gad, Raanan
    Sigal, Iliya
    Ringuette, Dene
    Koletar, Margaret
    Stefanovic, Bojana
    Levi, Ofer
    OPTICAL TECHNIQUES IN NEUROSURGERY, NEUROPHOTONICS, AND OPTOGENETICS II, 2015, 9305
  • [2] Investigation of psoriasis skin tissue by label-free multi-modal imaging: a case study on a phototherapy-treated patient
    Agarwal, Nisha Rani
    Pour, Masoumeh Dowlatshahi
    Vandikas, Maria Siekkeri
    Neittaanmaki, Noora
    Osmancevic, Amra
    Malmberg, Per
    PSORIASIS-TARGETS AND THERAPY, 2019, 9 : 43 - 57
  • [3] Epi-detecting label-free multimodal imaging platform using a compact diode-pumped femtosecond solid-state laser
    Andreana, Marco
    Le, Tuan
    Hansen, Anders K.
    Verhoef, Aart J.
    Jensen, Ole B.
    Andersen, Peter E.
    Slezak, Paul
    Drexler, Wolfgang
    Fernandez, Alma
    Unterhuber, Angelika
    JOURNAL OF BIOMEDICAL OPTICS, 2017, 22 (09)
  • [4] Label-free multi-photon imaging using a compact femtosecond fiber laser mode-locked by carbon nanotube saturable absorber
    Kieu, K.
    Mehravar, S.
    Gowda, R.
    Norwood, R. A.
    Peyghambarian, N.
    BIOMEDICAL OPTICS EXPRESS, 2013, 4 (10): : 2187 - 2195
  • [5] To label or not: the need for validation in label-free imaging
    Szulczewski, Joseph M.
    Yesilkoy, Filiz
    Ulland, Tyler K.
    Bartels, Randy
    Millis, Bryan A.
    Boppart, Stephen A.
    Eliceiri, Kevin W.
    JOURNAL OF BIOMEDICAL OPTICS, 2024, 29
  • [6] Supercontinuum fiber laser-based coherent anti-Stokes Raman scattering microscopy for label-free chemical imaging
    Wang, Nan
    Zhang, Jiaojiao
    Zhou, Wangting
    Liu, Siting
    Li, Jing
    Xu, Xinyi
    Zeng, Qi
    Li, Yong
    Zhu, Shouping
    Chen, Xueli
    JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES, 2022, 15 (04)
  • [7] Epi-detected quadruple-modal nonlinear optical microscopy for label-free imaging of the tooth
    Wang, Zi
    Zheng, Wei
    Hsu, Chin-Ying Stephen
    Huang, Zhiwei
    APPLIED PHYSICS LETTERS, 2015, 106 (03)
  • [8] Label-free imaging of fatty acid content within yeast samples
    Garrett, N.
    Moger, J.
    IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES XI, 2013, 8587
  • [9] Intravital imaging by simultaneous label-free autofluorescence-multiharmonic microscopy
    You, Sixian
    Tu, Haohua
    Chaney, Eric J.
    Sun, Yi
    Zhao, Youbo
    Bower, Andrew J.
    Liu, Yuan-Zhi
    Marjanovic, Marina
    Sinha, Saurabh
    Pu, Yang
    Boppart, Stephen A.
    NATURE COMMUNICATIONS, 2018, 9
  • [10] Label-Free Imaging of Lipid-Droplet Intracellular Motion in Early Drosophila Embryos Using Femtosecond-Stimulated Raman Loss Microscopy
    Dou, Wei
    Zhang, Delong
    Jung, Yookyung
    Cheng, Ji-Xin
    Umulis, David M.
    BIOPHYSICAL JOURNAL, 2012, 102 (07) : 1666 - 1675
12345