New anthracycline metabolites produced by the aklavinone 11-hydroxylase gene in Streptomyces galilaeus ATCC 31133

被引：27

作者：

Kim, HS

Hong, YS

Kim, YH

Yoo, OJ

Lee, JJ

机构：

[1] KIST,KOREA RES INST BIOSCI & BIOTECHNOL,MICROBIAL CHEM RES GRP,TAEJON 305600,SOUTH KOREA

[2] KOREA ADV INST SCI & TECHNOL,DEPT BIOL SCI,YUSUNG GU,TAEJON 305701,SOUTH KOREA

来源：

JOURNAL OF ANTIBIOTICS | 1996年 / 49卷 / 04期

关键词：

D O I：

10.7164/antibiotics.49.355

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Transformation of Streptomyces galilaeus ATCC 31133 with the aklavinone 11-hydroxylase gene (dnr F) resulted in the production of many red pigments. The new metabolites were purified and their structures were determined as 11-hydroxylated aclacinomycin A, B and T by spectral analysis. This result indicated that the dnrF was stably expressed in the strain S. galilaeus ATCC 31133 to give rise to hybrid aclacinomycins. In addition, a new aclacinomycin analog named 11-hydroxyaclacinomycin X was isolated from the same culture. Its structure was elucidated as 2'''amino-11-hydroxyaclacinomycin Y. It showed strong cytotoxicity against several human tumor cell lines, especially leukemia and melanoma cell lines.

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页码：355 / 360

页数：6

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