Arabidopsis Phosphoglycerate Dehydrogenase1 of the Phosphoserine Pathway Is Essential for Development and Required for Ammonium Assimilation and Tryptophan Biosynthesis

被引：121

作者：

Benstein, Ruben Maximilian ^{[1
]}

Ludewig, Katja ^{[1
]}

Wulfert, Sabine ^{[1
]}

Wittek, Sebastian ^{[1
]}

Gigolashvili, Tamara ^{[1
]}

Frerigmann, Henning ^{[1
]}

Gierth, Markus ^{[1
]}

Fluegge, Ulf-Ingo ^{[1
]}

Krueger, Stephan ^{[1
]}

机构：

[1] Univ Cologne, Cologne Bioctr, Bot Inst 2, D-50674 Cologne, Germany

来源：

PLANT CELL | 2013年 / 25卷 / 12期

关键词：

AMINO-ACIDS; SERINE BIOSYNTHESIS; 3-PHOSPHOGLYCERATE DEHYDROGENASE; ESCHERICHIA-COLI; D-3-PHOSPHOGLYCERATE DEHYDROGENASE; GLUCOSINOLATE BIOSYNTHESIS; AUXIN BIOSYNTHESIS; MERISTEM GROWTH; HIGHER-PLANTS; THALIANA;

D O I：

10.1105/tpc.113.118992

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In plants, two independent serine biosynthetic pathways, the photorespiratory and glycolytic phosphoserine (PS) pathways, have been postulated. Although the photorespiratory pathway is well characterized, little information is available on the function of the PS pathway in plants. Here, we present a detailed characterization of phosphoglycerate dehydrogenases (PGDHs) as components of the PS pathway in Arabidopsis thaliana. All PGDHs localize to plastids and possess similar kinetic properties, but they differ with respect to their sensitivity to serine feedback inhibition. Furthermore, analysis of pgdh1 and phosphoserine phosphatase mutants revealed an embryo-lethal phenotype and PGDH1-silenced lines were inhibited in growth. Metabolic analyses of PGDH1-silenced lines grown under ambient and high CO2 conditions indicate a direct link between PS biosynthesis and ammonium assimilation. In addition, we obtained several lines of evidence for an interconnection between PS and tryptophan biosynthesis, because the expression of PGDH1 and PHOSPHOSERINE AMINOTRANSFERASE1 is regulated by MYB51 and MYB34, two activators of tryptophan biosynthesis. Moreover, the concentration of tryptophan-derived glucosinolates and auxin were reduced in PGDH1-silenced plants. In essence, our results provide evidence for a vital function of PS biosynthesis for plant development and metabolism.

引用

页码：5011 / 5029

页数：19

共 91 条

[71] A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos [J].