Simultaneous production of multi-functional peptides by pancreatic hydrolysis of bovine casein in an enzymatic membrane reactor via combinational chromatography

被引：31

作者：

Wu, Shufen ^{[1
]}

Qi, Wei ^{[1
,2
,3
]}

Li, Tonghe ^{[1
]}

Lu, Dan ^{[1
]}

Su, Rongxin ^{[1
,2
,3
]}

He, Zhimin ^{[1
,2
]}

机构：

[1] Tianjin Univ, Sch Chem Engn & Technol, Chem Engn Res Ctr, Tianjin 300072, Peoples R China

[2] Tianjin Univ, State Key Lab Chem Engn, Tianjin 300072, Peoples R China

[3] Tianjin Univ, Tianjin Key Lab Membrane Sci & Desalinat Technol, Tianjin 300072, Peoples R China

来源：

FOOD CHEMISTRY | 2013年 / 141卷 / 03期

关键词：

ACEIPs; Casein phosphopeptides; Antimicrobial peptides; Enzymatic membrane reactor; Bovine casein; ANGIOTENSIN-CONVERTING ENZYME; INHIBITORY PEPTIDES; ANTIHYPERTENSIVE PEPTIDES; STRUCTURAL-ANALYSIS; IDENTIFICATION; MILK; PROTEINASE;

D O I：

10.1016/j.foodchem.2013.05.050

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Three bioactive peptides, angiotensin-converting enzyme-inhibitory peptides (ACEIPs), casein phosphopeptides (CPPs) and antimicrobial peptides (AMPs), were simultaneously prepared from casein by pancreatic hydrolysis via an enzymatic membrane reactor (EMR) and combinational chromatography. The reaction was performed at 37 degrees C and pH 8.0 for 3 h followed by ultrafiltration. ACEIPs were purified by size exclusion chromatography (SEC) from permeate fractions with molecular weight (MW) below 1 kDa. Concurrently, strong cation exchange high-performance liquid chromatography (SCE-HPLC) was used to isolate CPPs and AMPs from retentate fractions ranging from 1 kDa and 5 kDa. Following reverse-phase high performance liquid chromatography-electrospray ionization tandem mass spectrometry (RP-HPLC-ESI-MS/MS) analysis, potential ACEIPs and a total of 34 CPPs were identified (18 corresponded to alpha(s1)-casein, 2 to alpha(s2)-casein, 10 to beta-casein and 4 to kappa-casein). Additionally, the ACEIPs in fraction B from SEC had the highest ACE-inhibiting-activity (73.5% in 1.0 mg/ml), while total concentrations of AMPs were directly proportional to overall antibacterial activity. Uniquely, fraction 5 from SCE exhibited the highest activity against Staphylococcus aureus (97.49% in 1.0 mg/ml). (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：2944 / 2951

页数：8