Chronic subacromial impingement leads to supraspinatus muscle functional and morphological changes: Evaluation in a murine model

被引:11
作者
Croen, Brett J. [1 ,2 ]
Carballo, Camila B. [1 ]
Wada, Susumu [1 ]
Zhang, Xueying [1 ]
Patel, Saral [1 ]
Deng, Xiang-Hua [1 ]
Rodeo, Scott A. [1 ]
机构
[1] Hosp Special Surg, Dept Orthopaed Surg, 525 E 71st St,1st Floor, New York, NY 10021 USA
[2] Drexel Univ, Coll Med, Philadelphia, PA 19104 USA
基金
美国国家卫生研究院;
关键词
fatty infiltration; gait analysis; muscle atrophy; shoulder impingement model; supraspinatus tendinopathy; MOUSE MODEL; SHOULDER; PAIN; DISORDERS; DIAGNOSIS; OUTCOMES;
D O I
10.1002/jor.24964
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Muscle atrophy and fatty infiltration have been directly correlated with higher rates of incomplete or failed healing following surgical repair of the rotator cuff. The purpose of this study was to evaluate clinically relevant functional and morphological changes in the supraspinatus muscle at various time points in this model of rotator cuff tendinopathy. Subacromial impingement was induced in 47, male C57BL/6 mice (total 94 limbs) by implantation of a metal clip in the subacromial space. Specimens were evaluated at 4, 6, and 12 weeks postoperatively. Gait analysis was used to measure various kinematic parameters. Supraspinatus muscle wet weight, histology, and quantitative reverse-transcription polymerase chain reaction analysis of genes related to muscle atrophy and adipogenesis were performed to characterize the structural, cellular, and molecular changes. Muscle atrophy and fatty infiltration was evident beginning at 6 weeks, with progression out to 12 weeks. Gait analysis identified significant functional changes in many aspects of gait and abnormal stance tracing as early as 4 weeks, verifying alterations in upper extremity function. We have demonstrated that clinically relevant changes to the supraspinatus muscle are seen starting 6 weeks after induction of subacromial impingement. Furthermore, the gait analysis provides key functional outcome measurements that may be useful for future evaluation of new therapeutic strategies.
引用
收藏
页码:2243 / 2251
页数:9
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